Department of Internal Medicine, Kantonsspital Graubünden and Regional Blood Bank, Swiss Red Cross, Chur, Switzerland.
Clin Hemorheol Microcirc. 2011;49(1-4):451-61. doi: 10.3233/CH-2011-1495.
We studied the influence of metabolic depletion on red blood cell (RBC) aggregability, which is a determinant of blood flow. Heparinized blood was stored at room temperature for 0, 24, and 48 h. RBCs were washed twice and resuspended in Tris-buffer containing 3% dextran 70 (hematocrit 30%). Suspension viscosities were measured at 37 °C and shear rates of 37.6 and 0.1 s(-1), RBC aggregability was analysed by the sedimentation rate, direct microscopic visualization and a Myrenne aggregometer. RBCs in autologous plasma showed an increasing echinocytic shape transformation, which was reversible in buffer. The viscosities of RBC suspensions in buffer remained unchanged at both low (0.1 s(-1)) and high shear rate (37.6 s(-1)), the latter result indicating an unchanged RBC deformability. RBC aggregability decreased: The RBC sedimentation rates were 40.7 ± 5.0, 29.3 ± 13.4, and 13.3 ± 11.2 mm/h (p < 0.001) at 0, 24, and 48 h, respectively, which correlated well with the visual aggregability index and the Myrenne aggregation parameters M and M1. We conclude that metabolic depletion for 48 h leads to RBC swelling and a reversible echinocytic shape transformation. These ATP-depleted, but normally shaped RBCs had a decreased aggregability. In contrast to all other methods used, low shear viscosity was inaccurate and should not be used to test RBC aggregability.
我们研究了代谢耗竭对红细胞（RBC）聚集性的影响，红细胞聚集性是血流的决定因素。肝素化血液在室温下储存 0、24 和 48 小时。将 RBC 洗涤两次并重新悬浮在含有 3%右旋糖酐 70（红细胞压积 30%）的 Tris 缓冲液中。在 37°C 和 37.6 和 0.1 s(-1)的剪切速率下测量悬浮液的粘度，通过沉降率、直接显微镜观察和 Myrenne 聚集仪分析 RBC 聚集性。自体血浆中的 RBC 表现出逐渐的棘状变形，这种变形在缓冲液中是可逆的。缓冲液中 RBC 悬浮液的粘度在低剪切率（0.1 s(-1))和高剪切率（37.6 s(-1))下均保持不变，后者表明 RBC 变形性不变。RBC 聚集性降低：RBC 沉降率分别为 40.7 ± 5.0、29.3 ± 13.4 和 13.3 ± 11.2 mm/h（p < 0.001），在 0、24 和 48 小时时，与视觉聚集指数和 Myrenne 聚集参数 M 和 M1 相关性良好。我们得出结论，代谢耗竭 48 小时会导致 RBC 肿胀和可逆的棘状变形。这些缺乏 ATP 的，但形状正常的 RBC 聚集性降低。与使用的所有其他方法相反，低剪切粘度不准确，不应用于测试 RBC 聚集性。
