Kim Gun-Hwa, Park Edmond Changkyun, Han Jin-Kwan
Division of Life Science, Korea Basic Science Institute, Daejeon, Republic of Korea.
Methods Mol Biol. 2012;839:79-89. doi: 10.1007/978-1-61779-510-7_7.
The Wnt/planar cell polarity (PCP) signaling pathway plays a critical role in wing, eye, neural tube defects, and sensory bristle development of Drosophila and vertebrate development. Recently, the Wnt/PCP pathway has been known to regulate convergent extension (CE) movements that are essential for establishing the three germ layers and body axis during early vertebrate development. Here, we describe detailed practical procedures required for the particular studies in Xenopus CE movements.
Wnt/平面细胞极性(PCP)信号通路在果蝇的翅膀、眼睛、神经管缺陷以及感觉刚毛发育和脊椎动物发育中起着关键作用。最近,人们已经知道Wnt/PCP通路可调节汇聚延伸(CE)运动,而这种运动对于脊椎动物早期发育过程中三个胚层和身体轴的建立至关重要。在此,我们描述了非洲爪蟾CE运动特定研究所需的详细实际操作步骤。