PEG 连接的鸟苷缩醛缀合物用于修饰 RNA 的酶合成。

PEG-tethered guanosine acetal conjugates for the enzymatic synthesis of modified RNA.

机构信息

Institute of Pharmacy and Molecular Biotechnology, Heidelberg University, Im Neuenheimer Feld 364, Heidelberg 69120, Germany.

出版信息

Biochem Biophys Res Commun. 2012 Jan 27;417(4):1224-6. doi: 10.1016/j.bbrc.2011.12.114. Epub 2012 Jan 2.

DOI:10.1016/j.bbrc.2011.12.114

PMID:22230383

Abstract

A short and efficient synthetic route to guanosine monophosphate conjugates connected to a protected aldehyde group via a flexible polydisperse polyethylene glycol linker is described. These conjugates are enzymatically incorporated into RNA transcripts with 52% yield. The resulting aldehyde-modified RNA gave full conversion in the reaction with biotin hydrazide, demonstrating the utility of the method.

摘要

描述了一种短而高效的合成途径，可将通过柔性多分散性聚乙二醇接头连接到保护醛基的鸟苷一磷酸缀合物。这些缀合物以 52%的产率酶促掺入 RNA 转录物中。所得醛修饰的 RNA 与生物素酰肼完全转化，证明了该方法的实用性。