一种用于氨基配体共价结合的先进亲和膜。
An advanced affinity membrane for covalent binding of amino ligands.
作者信息
Pemawansa K P, Heisler M D, Blackwell S L, Cymes L, Mahalak K L, Kraus M A
出版信息
Biotechniques. 1990 Sep;9(3):352-7.
We report here an advanced, chemically active and yet hydrolytically stable microporous membrane which allows permanent covalent binding of amino ligands such as proteins. Rapid, single-step immobilizations produce a high density of immobilized ligands. Surface chemistry of the membrane is specifically designed to have extremely low nonspecific binding. Binding characteristics of the UltraBind membrane, various immobilization techniques and optimum immobilization conditions for diagnostic immunoassays are described.
我们在此报告一种先进的、具有化学活性且水解稳定的微孔膜，它允许氨基配体（如蛋白质）进行永久性共价结合。快速的单步固定化可产生高密度的固定化配体。该膜的表面化学经过特殊设计，具有极低的非特异性结合。本文描述了UltraBind膜的结合特性、各种固定化技术以及诊断免疫测定的最佳固定化条件。
Zotero 插件