Wang Han-Tsung, Chen I-Hung, Hsu Jih-Tay
Department of Animal Science, Chinese Culture University, Taipei, Taiwan.
Biosci Biotechnol Biochem. 2012;76(1):34-41. doi: 10.1271/bbb.110348. Epub 2012 Jan 7.
The characteristics of a bacteriocin from Ruminococcus albus 7 and its potential as an antibiotic alternative were examined in this study. The addition of 3 µM 3-phenylpropanoic acid (PPA) and 0.2% Tween 80 to the culturing medium improved bacteriocin production by 2.5-fold. Native polyacrylamide gel electrophoresis of the antagonistically active gel filtration fraction established that the molecular weight of the R. albus 7 bacteriocin was approximately 36 kDa. The bacteriocin was sensitive to pepsin, protease, and pancreatin, and was inactivated by heating at 65 °C for 1 h. Simulating in vitro avian digestion decreased the antagonistic activity by 74.7%, but the addition of 1% bovin serum albumin restored 13% of the lost antagonistic activity. Following ion-exchange purification, the bacteriocin had sufficient antagonistic activity against five tested pathogenic strains, but the addition of a protectant is necessary for utilization of bacteriocin of R. albus 7 as an antibiotic alternative in animal feed.
本研究检测了来自白色瘤胃球菌7的一种细菌素的特性及其作为抗生素替代品的潜力。向培养基中添加3 µM 3-苯丙酸（PPA）和0.2%吐温80可使细菌素产量提高2.5倍。对具有拮抗活性的凝胶过滤组分进行天然聚丙烯酰胺凝胶电泳，确定白色瘤胃球菌7细菌素的分子量约为36 kDa。该细菌素对胃蛋白酶、蛋白酶和胰酶敏感，在65℃加热1小时会失活。模拟体外禽类消化使拮抗活性降低了74.7%，但添加1%牛血清白蛋白可恢复13%丧失的拮抗活性。经过离子交换纯化后，该细菌素对五种受试病原菌具有足够的拮抗活性，但在动物饲料中使用白色瘤胃球菌7细菌素作为抗生素替代品时，添加一种保护剂是必要的。
