Kondo Machiko, Yoshida Yuki, Miyata Chika, Fujiwara Satsuki, Nakajima Yoshihiro, Hirai Hiroyuki, Takayama Seiji, Isogai Akira, Che Fang-Sik
Graduate School of Bio-Science, Nagahama Institute of Bio-Science and Technology, Nagahama, Shiga, Japan.
Biosci Biotechnol Biochem. 2012;76(1):129-38. doi: 10.1271/bbb.110597. Epub 2012 Jan 7.
The immune system of plants consists of two main arms, pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI) and effector-triggered immunity (ETI). The multiple effectors that trigger ETI are translocated into plant cells by the type III secretion system (T3SS) of pathogenic bacteria. The rice-avirulent N1141 strain of Acidovorax avenae causes ETI in rice, including hypersensitive response (HR) cell death. Sequence analysis indicated that the N1141 genome contains the hrp gene cluster (35.3 kb), including genes encoding the T3SS apparatus. The T3SS-defective N1141 mutant (NΔT3SS) did not cause HR cell death, suggesting that ETI is caused by translocation of effector proteins into rice cells via T3SS. Computational sequence analysis predicted that Lrp, HrpW, and HrpY are secreted by T3SS. The hrpY deletion mutant (NΔhrpY) did not cause ETI, suggesting that HrpY is an important effector of ETI in the interaction between A. avenae N1141 and rice.
植物的免疫系统由两个主要部分组成，即病原体相关分子模式（PAMP）触发的免疫（PTI）和效应子触发的免疫（ETI）。触发ETI的多种效应子通过病原菌的III型分泌系统（T3SS）转运到植物细胞中。燕麦嗜酸菌的水稻无毒N1141菌株在水稻中引发ETI，包括过敏反应（HR）细胞死亡。序列分析表明，N1141基因组包含hrp基因簇（35.3 kb），包括编码T3SS装置的基因。T3SS缺陷型N1141突变体（NΔT3SS）未引起HR细胞死亡，这表明ETI是由效应子蛋白通过T3SS转运到水稻细胞中引起的。计算序列分析预测Lrp、HrpW和HrpY由T3SS分泌。hrpY缺失突变体（NΔhrpY）未引起ETI，这表明HrpY是燕麦嗜酸菌N1141与水稻相互作用中ETI的重要效应子。
