枸杞提取物对MPP(+)诱导的秀丽隐杆线虫和PC12细胞神经毒性的保护作用
[Protective effects of Lycium barbarum extract against MPP(+) -induced neurotoxicity in Caenorhabditis elegans and PC12 cells].
作者信息
Yao Xiao-Li, Wu Wan-Ling, Zheng Min-Ying, Li Wei, Ye Cheng-Hui, Lu Xi-Lin
机构信息
Department of Neurology,The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510080, China.
出版信息
Zhong Yao Cai. 2011 Aug;34(8):1241-6.
OBJECTIVE
To investigate the neuroprotective effects of Lycium barbarum extract against MPP(+) -induced neurotoxicity in Caenorhabditis elegans and PC12 cells and its mechanism.
METHODS
Pretreated MPP(+) -induced nearotoxicity in C. elegans and PC12 cells with Lycium barbarum at different dosages. The viability and DA neurodegeneration was assessed in C. elegans selectively expressing green fluorescent protein (GFP) in DA neurons. PC12 cell damage was measured using MTT and nuclear morphology. Intracellular reactive oxygen species (ROS), mitochondrial membrane potential and total GSH were assessed.
RESULTS
Lycium barbarum extract protected against MPP(+) -induced loss of viability and DA neurodegeneration in C. elegans in a dose-dependent manner. Similar neuroprotection was replicated in MPP + PC12 cell model. Lycium barbarum extract attenuated MPP(+) -induced intracellular ROS accumulation, loss of mitochondrial membrane potential and restored total GSH levels in PCl2 cells.
CONCLUSIONS
Lycium barbarum extract protects against MPP(+) -induced neurotoxicity in C. elegans and PC12 cells and its machanism may be related to its antioxidative property and restoration of total GSH level.
目的
研究枸杞提取物对1-甲基-4-苯基吡啶离子（MPP(+)）诱导的秀丽隐杆线虫和PC12细胞神经毒性的神经保护作用及其机制。
方法
用不同剂量的枸杞预处理MPP(+)诱导的秀丽隐杆线虫和PC12细胞神经毒性。在多巴胺（DA）神经元中选择性表达绿色荧光蛋白（GFP）的秀丽隐杆线虫中评估其活力和DA神经元变性。使用MTT和细胞核形态测量PC12细胞损伤。评估细胞内活性氧（ROS）、线粒体膜电位和总谷胱甘肽（GSH）。
结果
枸杞提取物以剂量依赖的方式保护秀丽隐杆线虫免受MPP(+)诱导的活力丧失和DA神经元变性。在MPP + PC12细胞模型中也观察到类似的神经保护作用。枸杞提取物减轻了MPP(+)诱导的PC12细胞内ROS积累、线粒体膜电位丧失并恢复了总GSH水平。
结论
枸杞提取物可保护秀丽隐杆线虫和PC12细胞免受MPP(+)诱导的神经毒性，其机制可能与其抗氧化特性和总GSH水平的恢复有关。
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