Pansook Anusorn, Khonsue Wichase, Piyapattanakorn Sanit, Pariyanonth Putsatee
Biological Sciences Program, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand.
Zoolog Sci. 2012 Jan;29(1):54-9. doi: 10.2108/zsj.29.54.
A fragment (564 bp) of the mitochondrial cytochrome-b (Cyt-b) gene was studied for 73 individual rice field frogs (Hoplobatrachus rugulosus) from 18 geographical locations (populations) within Thailand. Sequence analysis revealed the presence of 12 haplotypes, with five haplotypes being represented in two or more populations, and the other seven being population-distinct haplotypes. Phylogenetic analysis by maximum parsimony, maximum likelihood, and neighbor joining analyses all placed the 12 haplotypes into two distinct and well-separated clades with high bootstrap support, reflecting the high sequence divergences between the clades (25.3-32.3%). The mountain ranges and the Isthmus of Kra are likely to have played important roles in hindering gene flow among H. rugulosus populations in Thailand. From the sequence divergence values, the two clades of H. rugulosus can be classified into two distinct species, and therefore, the strains of H. rugulosus bred in farm stocks should be restricted to a population of one clade so as to avoid cross breeding between the two clades.
对来自泰国18个地理位置（种群）的73只虎纹蛙（Hoplobatrachus rugulosus）的线粒体细胞色素b（Cyt-b）基因片段（564 bp）进行了研究。序列分析显示存在12种单倍型，其中5种单倍型在两个或更多种群中出现，另外7种为种群特异性单倍型。通过最大简约法、最大似然法和邻接法进行的系统发育分析均将这12种单倍型分为两个明显且分离良好的分支，自展支持率高，这反映了分支之间的高序列差异（25.3 - 32.3%）。山脉和克拉地峡可能在阻碍泰国虎纹蛙种群间的基因流动方面发挥了重要作用。根据序列差异值，虎纹蛙的两个分支可分为两个不同的物种，因此，养殖场养殖的虎纹蛙菌株应限制在一个分支的种群内，以避免两个分支之间的杂交。
