Faculty of Veterinary Medicine, Department of Physiological Sciences, Warsaw University of Life Sciences - WULS, Warsaw, Poland.
Vet Comp Oncol. 2012 Mar;10(1):2-15. doi: 10.1111/j.1476-5829.2011.00269.x. Epub 2011 May 2.
Canine mammary gland has been identified as a major site of the extrapituitary growth hormone (GH) production. This finding is linked to its role in tumourigenesis of the mammary gland. Our previous studies indicated the role of GH and GH receptor (GHR) in regulation of proliferation and apoptosis. Thus, we have optimized the ghr RNA interference method in canine mammary carcinoma cell line CMT-U27. We have analysed the effect of GHR reduction on the intracellular signalling and the cell cycle and apoptosis. The results showed that GHR reduction decreased the p-ERK1/2 expression and caused increase of apoptosis and decrease in number of cells at S and G2M phases. This study indicates that GHR besides proliferative effect promotes growth by increasing cell survival. It can tilt the balance between proliferation and death in cancer cells.
犬乳腺已被确定为脑垂体以外生长激素 (GH) 产生的主要部位。这一发现与其在乳腺肿瘤发生中的作用有关。我们之前的研究表明，GH 和 GH 受体 (GHR) 在调节增殖和凋亡中起作用。因此，我们在犬乳腺癌细胞系 CMT-U27 中优化了 ghr RNA 干扰方法。我们分析了 GHR 减少对细胞内信号转导和细胞周期及凋亡的影响。结果表明，GHR 减少降低了 p-ERK1/2 的表达，导致细胞凋亡增加，S 期和 G2M 期细胞数量减少。本研究表明，GHR 除了具有增殖作用外，还通过增加细胞存活来促进生长。它可以使癌细胞的增殖和死亡之间的平衡倾斜。
