Laboratory of Food Microbiology and Food Preservation, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, B-9000 Ghent, Belgium.
J Virol Methods. 2012 Apr;181(1):1-5. doi: 10.1016/j.jviromet.2012.01.001. Epub 2012 Jan 13.
Attempts were made to evaluate methods measuring the capsid integrity and/or functions of noroviruses (NoVs) following heat treatment. Intact viruses (Murine Norovirus-1 [MNV-1] and human NoV GII.4), virus like particles (VLPs) and P particles (expressed in vitro from the protruding domain of the viral capsid) of NoVs were used in this study. Following heat treatment, no significant difference of viral titer of MNV-1 versus NoV GII.4 was observed by RNase One RT-PCR or cell-binding RT-PCR, although cell-binding RT-PCR (to measure the capsid functions) revealed higher reductions than RNase One RT-PCR (to measure the capsid integrity). These results indicate that the function assay for receptor binding is more sensitive than the capsid integrity assay to measure the protected viral RNA. MNV-1 could be used as a surrogate for human NoVs by heat inactivation from the perspective of capsid integrity and/or functions. The heat resistance varied among different GI and GII NoV strains when their P particles were studied.
本研究采用了诺如病毒（NoV）经过热处理后的衣壳完整性和/或功能的检测方法。本研究使用了完整的病毒（鼠诺如病毒-1[MNV-1]和人诺如病毒 GII.4]）、病毒样颗粒（VLPs）和 P 颗粒（体外表达病毒衣壳突出结构域）。尽管细胞结合 RT-PCR（用于测量衣壳功能）比 RNase One RT-PCR（用于测量衣壳完整性）显示出更高的降低程度，但通过 RNase One RT-PCR 或细胞结合 RT-PCR 均未观察到 MNV-1 与 NoV GII.4 的病毒滴度有显著差异。这些结果表明，受体结合功能测定比衣壳完整性测定更能灵敏地测量保护的病毒 RNA。从衣壳完整性和/或功能的角度来看，MNV-1 可作为人类 NoV 的替代物进行热失活。当研究其 P 颗粒时，不同 GI 和 GII NoV 株之间的耐热性存在差异。
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