Department of Environmental Engineering, College of Life and Environmental Science, Shanghai Normal University, 200234, Shanghai, People's Republic of China.
Biodegradation. 2012 Jul;23(4):575-83. doi: 10.1007/s10532-012-9534-0. Epub 2012 Jan 20.
The mechanisms occurring in a photolytic circulating-bed biofilm reactor (PCBBR) treating 2,4,6-trichlorophenol (TCP) were investigated using batch experiments following three protocols: photodegradation alone (P), biodegradation alone (B), and intimately coupled photodegradation and biodegradation (P&B). Initially, the ceramic particles used as biofilm carriers rapidly adsorbed TCP, particularly in the B experiments. During the first 10 min, the TCP removal rate for P&B was equal to the sum of the rates for P and B, and P&B continued to have the greatest TCP removal, with the TCP concentration approaching zero only in the P&B experiments. When phenol, an easily biodegradable compound, was added along with TCP in order to promote TCP mineralization by means of secondary utilization, P&B was superior to P and B in terms of mineralization of TCP, giving 95% removal of chemical oxygen demand (COD). The microbial communities, examined by clone libraries, changed dramatically during the P&B experiments. Whereas Burkholderia xenovorans, a known degrader of chlorinated aromatics, was the dominant strain in the TCP-acclimated inoculum, it was replaced in the P&B biofilm by strains noted for biofilm formation and biodegrading non-chlorinated aromatics.
采用批式实验，研究了处理 2,4,6-三氯苯酚（TCP）的光解循环床生物膜反应器（PCBBR）中的作用机制，遵循三个方案：单独光降解（P）、单独生物降解（B）和紧密偶联的光降解和生物降解（P&B）。最初，用作生物膜载体的陶瓷颗粒迅速吸附 TCP，特别是在 B 实验中。在前 10 分钟内，P&B 的 TCP 去除率等于 P 和 B 的速率之和，并且 P&B 继续具有最大的 TCP 去除率，只有在 P&B 实验中 TCP 浓度才接近零。当在 TCP 中加入苯酚（一种易于生物降解的化合物）以通过二次利用促进 TCP 矿化时，P&B 在 TCP 矿化方面优于 P 和 B，化学需氧量（COD）去除率达到 95%。通过克隆文库检测微生物群落，在 P&B 实验过程中发生了巨大变化。虽然已知的氯代芳烃降解菌伯克霍尔德菌（Burkholderia xenovorans）是 TCP 驯化接种物中的优势菌株，但在 P&B 生物膜中，它被以生物膜形成和生物降解非氯代芳烃为特征的菌株所取代。
