Department of Obstetrics and Gynecology, Ankara University School of Medicine, Ankara, Turkey.
Arch Gynecol Obstet. 2012 Jul;286(1):173-8. doi: 10.1007/s00404-012-2236-3. Epub 2012 Jan 25.
PAX2 is a member of paired box gene family and expressed during development of urogenital system. This study aimed to evaluate PAX2 expression pattern in hyperplastic and malignant endometrial tissues in comparison to non-pathological endometrial changes and to investigate the presence of any correlation between the PAX2 expression and tumor behavior.
The study was performed on the archival material of 121 endometrial tissues including complex hyperplasia (n = 18), complex atypical hyperplasia (n = 20), and endometrioid type adenocarcinoma (n = 47) as study groups, and proliferative endometrium (n = 21) and atrophic endometrium (n = 16) as control groups. One representative block for each case was selected for immunohistochemical evaluation. Sections with 4μm thickness were cut from the blocks and incubated with PAX2 rabbit anti-human polyclonal antibody.
PAX2 nuclear staining was detected in all of the endometrial tissues. The mean percentages of PAX2 staining cells were 80.8, 96.7, 88.6, 92.7, and 99.2% with proliferative endometrium, atrophic endometrium, complex hyperplasia, complex atypical hyperplasia, and adenocarcinoma, respectively (Kruskal-Wallis; P < 0.001). The frequency of PAX2 staining increased as the pathology progressed in the manner of complex hyperplasia → complex atypical hyperplasia → adenocarcinoma. In cancer cases, there was no correlation between PAX2 expression levels and the stage, histological grade, myometrial invasion, and lymph node status.
PAX2 is expressed in hyperplastic and malignant endometrium as well as proliferative and atrophic endometrium. As the neoplastic lesion progresses from a premalignant state to endometrial cancer, PAX2 expression increases. These findings suggest that PAX2 may contribute to the development of endometrial cancer.
PAX2 是配对盒基因家族的成员，在泌尿生殖系统发育过程中表达。本研究旨在比较增生和恶性子宫内膜组织与非病理性子宫内膜改变的 PAX2 表达模式，并探讨 PAX2 表达与肿瘤行为之间的相关性。
该研究对 121 例子宫内膜组织的存档标本进行了研究，包括单纯性增生（n=18）、复杂性不典型增生（n=20）和子宫内膜样腺癌（n=47）为研究组，增殖性子宫内膜（n=21）和萎缩性子宫内膜（n=16）为对照组。为每个病例选择一个有代表性的块进行免疫组织化学评估。从块中切下 4μm 厚的切片，并用 PAX2 兔抗人多克隆抗体孵育。
所有子宫内膜组织均检测到 PAX2 核染色。PAX2 染色细胞的平均百分比分别为 80.8%、96.7%、88.6%、92.7%和 99.2%，分别为增殖性子宫内膜、萎缩性子宫内膜、单纯性增生、复杂性不典型增生和腺癌（Kruskal-Wallis；P<0.001）。PAX2 染色频率随着病理学从单纯性增生→复杂性不典型增生→腺癌的进展而增加。在癌症病例中，PAX2 表达水平与分期、组织学分级、肌层浸润和淋巴结状态之间无相关性。
PAX2 在增生和恶性子宫内膜以及增殖和萎缩性子宫内膜中均有表达。随着肿瘤从癌前状态进展为子宫内膜癌，PAX2 表达增加。这些发现表明 PAX2 可能有助于子宫内膜癌的发生。
