Department of Radiation Oncology, Shands Cancer Center, University of Florida, Gainesville, Gainesville, FL 32610-0385, USA.
Cytokine. 2012 May;58(2):169-77. doi: 10.1016/j.cyto.2011.12.023. Epub 2012 Jan 25.
To determine the plasma concentrations of acute responding cytokines/chemokines following 9-Gy ionizing radiation in C57BL/6 (radiation tolerant) and C3H/HeN (radiation sensitive) murine strains.
Mice (5/group) received 9-Gy total body irradiation (TBI), and the plasma from each mouse was collected at 6h or 1, 2, 4, or 10 days after TBI. A multiplex bead array was used to assess the levels of 32 cytokines/chemokines in plasma to determine their common and strain-specific temporal responses.
The plasma levels of five cytokines/chemokines (Axl, FasL, ICAM-1, TARC, and TSLP) were beyond the detectable level. Five (VEGF, IL-2, IL-5, IL-17, and CD30) were unaffected by irradiation in either strain. Temporal patterns were similar in both murine strains for 10 of the cytokines tested, including G-CSF, IL-6, TCA-3, MCP-1, MIP-1γ, KC, CXCL 13, CXCL 16, MDC, and TIMP-1; the other 12 molecules (GM-CSF, IL-3, SCF, IL-1β, IL-4, IL-10, IL-12p70, MIP-1α, Eotaxin, TNF-α, sTNF-R1, and CD40) showed strain-specific response patterns. While a number of cytokines had temporal response patterns following TBI, the strains exhibited quantitatively different results.
The levels of 27 of the 32 plasma cytokines measured indicate the following: (1) different cytokine concentrations and temporal patterns in the two strains may partly explain different radiation sensitivities and sequelae following irradiation; (2) many of the cytokines/chemokines exhibit similar temporal responses in the two strains. These responses suggest the potential value of using a panel of cytokine/chemokine temporal patterns for radiation dosimetry. Although radiation doses will be difficult to quantitate due to the large variation in levels and temporal responses exhibited in the two murine strains, serial measurements of cytokines might help identify subjects exposed to radiation.
测定 C57BL/6（辐射耐受）和 C3H/HeN（辐射敏感）两种鼠种在接受 9-Gy 全身照射（TBI）后急性反应细胞因子/趋化因子的血浆浓度。
每组 5 只小鼠接受 9-Gy 全身 TBI，在 TBI 后 6h 或 1、2、4 或 10 天收集每只小鼠的血浆。采用多重微珠阵列检测血浆中 32 种细胞因子/趋化因子的水平，以确定它们的共同和株特异性时间反应。
五种细胞因子/趋化因子（Axl、FasL、ICAM-1、TARC 和 TSLP）的血浆水平超出了可检测水平。在两种鼠种中，五种细胞因子（VEGF、IL-2、IL-5、IL-17 和 CD30）不受照射影响。在所测试的 10 种细胞因子中，两种鼠种的时间模式相似，包括 G-CSF、IL-6、TCA-3、MCP-1、MIP-1γ、KC、CXCL 13、CXCL 16、MDC 和 TIMP-1；其他 12 种分子（GM-CSF、IL-3、SCF、IL-1β、IL-4、IL-10、IL-12p70、MIP-1α、Eotaxin、TNF-α、sTNF-R1 和 CD40）表现出株特异性反应模式。虽然许多细胞因子在 TBI 后具有时间反应模式，但两种品系表现出定量不同的结果。
在所测量的 32 种血浆细胞因子中，有 27 种表明：（1）两种品系中的不同细胞因子浓度和时间模式可能部分解释了照射后不同的辐射敏感性和后遗症；（2）两种品系中的许多细胞因子/趋化因子表现出相似的时间反应。这些反应表明，使用细胞因子/趋化因子时间模式的组合进行辐射剂量测定具有潜在价值。尽管由于两种鼠种中水平和时间反应的差异很大，辐射剂量难以定量，但细胞因子的连续测量可能有助于识别暴露于辐射的个体。
