磷酸浓度和蚀刻时间对接受血液透析的尿毒症患者牙釉质和牙本质组织的影响：AFM 研究。

Effects of phosphoric acid concentration and etching duration on enamel and dentin tissues of uremic patients receiving hemodialysis: an AFM study.

机构信息

Conservative Dentistry Department, Faculty of Dentistry, Mansoura University, Mansoura, Egypt.

出版信息

J Adhes Dent. 2012 Jun;14(3):215-21. doi: 10.3290/j.jad.a22421.

DOI:10.3290/j.jad.a22421

PMID:22282744

Abstract

PURPOSE

Using atomic force microscopy (AFM), the purpose was to investigate the effect of phosphoric acid (H3PO4) concentration and etching duration on surface roughness (Ra) and micromorphology of enamel and dentin substrates of uremic patients receiving hemodialysis.

MATERIALS AND METHODS

Sixty-three enamel and dentin slabs were prepared from 42 sound natural molars collected from uremic patients under hemodialysis and mechanically polished up to 4000-grit roughness. Nine slabs of each substrate were not etched (control group). The remaining slabs were divided into two experimental groups (n = 27) according to etchant concentration (37% or 42%). In each group, 9 specimens were etched for 15, 30, and 60 s. The surface morphology of the control and test specimens was examined by AFM operated in "contact" mode. The obtained Ra was subjected to statistical analysis.

RESULTS

Statistical analysis showed that increasing the duration of acid application (irrespective of acid concentration and type of substrate) significantly increased the amount of Ra. H3PO4 at a concentration of 42% was more effective at producing Ra than was H3PO4 at 37% for all 3 application times. The Ra quantity produced by H3PO4 at 42% was time specific, with 15 s being significantly less effective than 30 or 60 s. However, 60 s was significantly better than 30.

CONCLUSION

The findings of this study support the use of 42% H3PO4 for etching uremic hard tooth tissues for 60 s.

摘要

目的

使用原子力显微镜（AFM），研究磷酸（H3PO4）浓度和蚀刻时间对接受血液透析的尿毒症患者牙釉质和牙本质基质表面粗糙度（Ra）和微观形态的影响。

材料和方法

从 42 名接受血液透析的尿毒症患者的 42 颗天然磨牙中制备了 63 个牙釉质和牙本质板，并机械研磨至 4000 粒度粗糙度。每个基质的 9 个板未经蚀刻（对照组）。其余的板根据蚀刻剂浓度（37%或 42%）分为两个实验组（n = 27）。在每个组中，9 个样本分别蚀刻 15、30 和 60 s。使用“接触”模式操作的 AFM 检查对照和测试样本的表面形态。对获得的 Ra 进行统计分析。

结果

统计分析表明，无论酸的浓度和基质类型如何，增加酸的作用时间都会显著增加 Ra 的量。对于所有 3 种应用时间，浓度为 42%的 H3PO4 比浓度为 37%的 H3PO4 更有效地产生 Ra。42% H3PO4 产生的 Ra 量是时间特异性的，15 s 的效果明显低于 30 s 或 60 s。然而，60 s 明显优于 30 s。