利用粪便样本对 APCmin/+ 小鼠进行非侵入性基因分型的方法。

A method for non-invasive genotyping of APCmin/+ mice using fecal samples.

机构信息

Nutrigenomics and Nutrigenetics, CSIRO- Food and Nutritional Sciences, Adelaide, South Australia.

出版信息

Biol Proced Online. 2012 Jan 30;14(1):1. doi: 10.1186/1480-9222-14-1.

DOI:10.1186/1480-9222-14-1

PMID:22284906

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3293049/

Abstract

The APCmin/+ mouse is commonly used in cancer research and is just one of many genetically altered models that is currently being developed. With high numbers of breeding programs, it is important to have a simple method that can be used to genotype the mice non-invasively. Here we report a reproducible method for genotyping mice with DNA extracted from fecal samples. Comparison of fecal results with those obtained from intestinal tissue DNA and clinical outcome (presence/absence of tumors) showed this technique to have 100% accuracy. This non-invasive method of genotyping may be applied to other transgenic mouse models.

摘要

APCmin/+ 小鼠常用于癌症研究，它只是目前正在开发的众多基因改变模型之一。由于繁殖计划数量众多，因此需要有一种简单的方法来对小鼠进行非侵入性基因分型。在这里，我们报告了一种从粪便样本中提取 DNA 对小鼠进行基因分型的可重复方法。粪便结果与从肠道组织 DNA 获得的结果和临床结果（肿瘤的存在/缺失）的比较表明，该技术的准确性达到 100%。这种非侵入性的基因分型方法可应用于其他转基因小鼠模型。