Wagner H E, Thomas P, Wolf B C, Zamcheck N, Jessup J M, Steele G D
Department of Surgery, New England Deaconess Hospital, Harvard Medical School, Boston, Mass.
Invasion Metastasis. 1990;10(5):253-66.
We characterized the tumorigenic and metastatic potential of a poorly differentiated, non-CEA-producing colon cancer cell line, MIP-101, after injection at different sites in athymic mice. After subcutaneous and intrasplenic injection tumor grew locally in 100 and 50%, respectively, but no metastases were found, even after intravenous injection. Intraperitoneal implantation, however, resulted in a high tumor take (10/10) and subsequent liver colonization (8/10 mice). Exogenous CEA prior to intrasplenic injection induced metastasis in 7/8 mice (in 2 mice to the liver and in 5 mice to the lung). Intrasplenic injection of CX-1, a good CEA producer, resulted in hepatic metastases in 100% of the animals. These data suggest a direct or indirect role of CEA in the metastatic process. We conclude that MIP-101 has a high tumorigenic and invasive potential but a low metastatic proclivity, except when grown in the peritoneum, and that pretreatment of tumor-bearing animals with CEA affects the metastatic proclivity.
我们在无胸腺小鼠的不同部位注射低分化、不产生癌胚抗原（CEA）的结肠癌细胞系MIP-101后，对其致瘤性和转移潜能进行了表征。皮下注射和脾内注射后，肿瘤分别在100%和50%的小鼠中局部生长，但即使静脉注射后也未发现转移。然而，腹腔内植入导致高肿瘤接种成功率（10/10）以及随后的肝脏定植（8/10小鼠）。脾内注射前给予外源性CEA可使7/8小鼠发生转移（2只小鼠转移至肝脏，5只小鼠转移至肺）。脾内注射CX-1（一种良好的CEA产生者）导致100%的动物发生肝转移。这些数据表明CEA在转移过程中具有直接或间接作用。我们得出结论，MIP-101具有高致瘤性和侵袭潜能，但转移倾向较低，除非在腹膜内生长，并且用CEA对荷瘤动物进行预处理会影响转移倾向。
