开发一种新的 HHV-6 定量实时 PCR 方法，并监测肝移植后 HHV-6 DNA 血症。
Development of a new quantitative real-time HHV-6-PCR and monitoring of HHV-6 DNAaemia after liver transplantation.
机构信息
Department of Virology, HUSLAB, Helsinki University Hospital and University of Helsinki, FIN-00029 HUS, Helsinki, Finland.
出版信息
J Virol Methods. 2012 Apr;181(1):25-36. doi: 10.1016/j.jviromet.2012.01.007. Epub 2012 Jan 24.
A quantitative HHV-6 PCR (qPCR) assay was developed and compared to an "in-house" qualitative PCR and to the commercial quantitative Argene CMV, HHV6, 7, 8 R-gene™ test. Clinical specimens consisting of 127 whole blood and 57 cerebrospinal fluid (CSF) specimens were tested using the two qPCRs and the qualitative PCR in parallel. When the qualitative PCR was used as a "gold standard," the sensitivities of the qPCRs for the blood samples were 86% for the "in-house" qPCR and 76% for the Argene's test and the specificities were 96% and 92%, respectively. With CSF specimens the sensitivities were 92% and 80% and the specificities 98% and 82%, respectively. Furthermore, the two qPCRs were compared in the monitoring of liver transplant patients and retrospectively correlated to HHV-6 antigenaemia. In total, 223 blood specimens were tested. HHV-6 antigenaemia had been found in 21/36 (58%) patients and HHV-6 DNAaemia was demonstrated in 18/36 (50%). Viral loads by the "in-house" test varied from 280 to 19700 copies/ml (median 1200) and by Argene's test from 120 to 24070 copies/ml (median 458). The correlation of viral loads between the two qPCRs was good (R=0.94, p<0.01). The new in-house test was found to be reliable for the detection and quantitation of HHV-6 DNA in clinical specimens.
开发了一种定量 HHV-6 PCR（qPCR）检测方法，并与内部定性 PCR 和商业定量 Argene CMV、HHV6、7、8 R-基因™ 检测进行了比较。使用这两种 qPCR 和定性 PCR 同时平行检测了由 127 份全血和 57 份脑脊液（CSF）标本组成的临床标本。当定性 PCR 作为“金标准”时，两种 qPCR 对血液样本的敏感性分别为内部 qPCR 的 86%和 Argene 测试的 76%，特异性分别为 96%和 92%。对于 CSF 标本，敏感性分别为 92%和 80%，特异性分别为 98%和 82%。此外，还比较了两种 qPCR 在肝移植患者监测中的应用，并回顾性地与 HHV-6 抗原血症相关联。共检测了 223 份血液标本。在 36 名患者中发现了 21 例（58%）HHV-6 抗原血症，在 36 名患者中发现了 18 例（50%）HHV-6 DNA 血症。内部测试的病毒载量从 280 到 19700 拷贝/ml（中位数为 1200），Argene 测试的病毒载量从 120 到 24070 拷贝/ml（中位数为 458）。两种 qPCR 之间的病毒载量相关性良好（R=0.94，p<0.01）。发现新的内部测试可用于可靠地检测和定量临床标本中的 HHV-6 DNA。
Zotero 插件