青叶胆及其根的抗乙肝病毒活性。
Anti-HBV activities of Streblus asper and constituents of its roots.
机构信息
Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, School of Environment and Resource of Guangxi Normal University, Guilin 541004, PR China.
出版信息
Fitoterapia. 2012 Jun;83(4):643-9. doi: 10.1016/j.fitote.2012.01.009. Epub 2012 Jan 27.
The extracts from leaves, heartwood, barks and roots of the Streblus asper were investigated for anti-HBV activities, separately. The results suggested that the MeOH extracts of the heartwood, barks, and roots exhibited good anti-HBV activities. Further investigations displayed that ethyl acetate and n-butanol soluble parts of their MeOH extracts showed more significant anti-HBV activities. Moreover, a new lignan, together with 11 known compounds, was isolated from n-butanol-soluble part of MeOH extract of the roots of S. asper. The structures were elucidated by spectroscopic methods, including 1D NMR ((1)H NMR, (13)C NMR), 2D NMR (HMQC, HMBC) and HR-EI-MS experiments. Compounds 1-3 were evaluated for their anti-HBV activities. Honokiol showed significant anti-HBV activity with IC(50) values of 3.14μM and 4.74μM for HBsAg and HBeAg with no cytotoxicity respectively, while lamivudine (3TC, positive control) exhibited weak anti-HBV activity with IC(50) values of 11.81μM and 25.80μM for HBsAg and HBeAg respectively.
从桃金娘科蒲桃属岗柃的叶、心材、树皮和根部分别提取，研究它们的抗乙肝病毒（HBV）活性。结果表明，心材、树皮和根的甲醇提取物均具有良好的抗 HBV 活性。进一步的研究表明，它们甲醇提取物的乙酸乙酯和正丁醇可溶部分显示出更显著的抗 HBV 活性。此外，从岗柃根的甲醇提取物的正丁醇可溶部分分离得到一个新的木脂素，以及 11 个已知化合物。通过包括 1D NMR ((1)H NMR、(13)C NMR)、2D NMR (HMQC、HMBC)和 HR-EI-MS 实验在内的光谱方法阐明了它们的结构。对化合物 1-3 进行了抗 HBV 活性评价。荜澄茄素表现出显著的抗 HBV 活性，HBsAg 和 HBeAg 的 IC(50)值分别为 3.14μM 和 4.74μM，且无细胞毒性，而拉米夫定（3TC，阳性对照）的抗 HBV 活性较弱，HBsAg 和 HBeAg 的 IC(50)值分别为 11.81μM 和 25.80μM。
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