Dept. of Biology, McMaster University, Hamilton, ON, Canada L8S 4K1.
Chemosphere. 2012 Jun;87(11):1208-14. doi: 10.1016/j.chemosphere.2012.01.018. Epub 2012 Feb 4.
We tested the use of whole-body and subcellular Cu residues (biologically-active (BAM) and inactive compartments (BIM)), of the oligochaete Lumbriculus variegatus to predict Cu toxicity in fresh water. The critical whole-body residue associated with 50% mortality (CBR(50)) was constant (38.2-55.6 μg g(-1) fresh wt.) across water hardness (38-117 mg L(-1) as CaCO(3)) and exposure times during the chronic exposure. The critical subcellular residue (CSR(50)) in metal-rich granules (part of BIM) associated with 50% mortality was approximately 5 μg g(-1) fresh wt., indicating that Cu bioavailability is correlated with toxicity:subcellular residue is a better predictor of Cu toxicity than whole-body residue. There was a strong correlation between the whole-body residue of L. variegatus (biomonitor) and survival of Chironomus riparius (relatively sensitive species) in a hard water Cu co-exposure. The CBR(50) in L. variegatus for predicting mortality of C. riparius was 29.1-45.7 μg g(-1) fresh wt., which was consistent within the experimental period; therefore use of Cu residue in an accumulator species to predict bioavailability of Cu to a sensitive species is a promising approach.
我们测试了多毛类环节动物正颤蚓的全身和亚细胞铜残留（生物活性（BAM）和非活性区室（BIM））在预测淡水铜毒性方面的应用。与 50%死亡率相关的关键全身残留（CBR（50））在水硬度（38-117mg/L 作为 CaCO（3））和慢性暴露期间的暴露时间范围内是恒定的（38.2-55.6μg/g 鲜重）。与 50%死亡率相关的关键亚细胞残留（CSR（50））在富含金属的颗粒（BIM 的一部分）中约为 5μg/g 鲜重，表明铜生物利用度与毒性相关：亚细胞残留是铜毒性的更好预测因子比全身残留。在硬水中铜共暴露下，正颤蚓的全身残留（生物监测器）与摇蚊幼虫（相对敏感物种）的存活率之间存在很强的相关性。正颤蚓的 CBR（50）预测摇蚊幼虫死亡率为 29.1-45.7μg/g 鲜重，在实验期间内是一致的；因此，使用积累物种中的铜残留来预测铜对敏感物种的生物利用度是一种很有前途的方法。
