Afanas'ev P K, Rubtsova G A, Shitova M V, Shaĭkhaev E G, Zhivotovskiĭ L A
Genetika. 2011 Nov;47(11):1473-80.
A set often microsatellite loci enabling fairly accurate identification of the chum salmon individuals from geographically distant groups was designed at the Laboratory of Genetic Identification, Vavilov Institute of General Genetics, Russian Academy of Sciences. However, identification of the individuals from closely located basins performed using these loci was not sufficiently precise. The present study was focused on the improvement of the resolution of the method through increasing the number microsatellite loci used. In this study, typing of additional microsatellite loci of chum salmon and evaluation of the change of the degree of identification with the increase of the number ofmicrosatellite loci used is described. It was shown that the identification accuracy permanently increased with the increase of the number of microsatellite markers used.
俄罗斯科学院瓦维洛夫普通遗传学研究所遗传鉴定实验室设计了一组十个微卫星基因座,能够相当准确地识别来自地理上遥远群体的大麻哈鱼个体。然而,使用这些基因座对来自位置相近流域的个体进行识别并不足够精确。本研究的重点是通过增加所使用的微卫星基因座数量来提高该方法的分辨率。在本研究中,描述了对大麻哈鱼额外微卫星基因座的分型以及随着所使用微卫星基因座数量增加对识别程度变化的评估。结果表明,随着所使用微卫星标记数量的增加,识别准确性持续提高。