Qin Wei, Zhong Xiang, Fan Jun-ming, Liu Xian-rong, Li Zi, Ma Xing-yi
Department of Nephrology, West China Hospital, Sichuan University, Chengdu 610041, China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2011 Nov;42(6):762-5.
To investigate the effect of methylation modification on the mRNA expression of Cosmc gene in peripheral B lymphocytes from IgA nephropathy (IgAN).
Biopsy identified 22 cases of IgAN patients and 20 cases of normal control were included. Peripheral B lymphocytes were isolated and were cultured with RPMI1640 medium, with LPS (12.5 microg/mL) as well as demethylation agent (5-AZA 0.1 micromol/L, 0.5 micromol/ L and 1.0 micromol/L) for 72 hours, respectively. Expression level of Cosmc gene was measured using real-time PCR.
The mRNA expression level of Cosmc gene in IgAN patients was significantly lower than that of control (P<0.05). The Cosmc gene expression level increased dramatically after RPMI1640 treatment (P<0.05), however, LPS could apparently reverse this effect (P<0.05). De-methylation modification up regulated the Cosmc gene expression significantly (P<0.05).
De-methylation modification could effectively reverse the repressed Comsc gene mRNA expression caused by external suppressors.
探讨甲基化修饰对IgA肾病（IgAN）患者外周血B淋巴细胞中Cosmc基因mRNA表达的影响。
经活检确定纳入22例IgAN患者及20例正常对照。分离外周血B淋巴细胞，分别用RPMI1640培养基、脂多糖（LPS，12.5μg/mL）以及去甲基化剂（5 - 氮杂胞苷0.1μmol/L、0.5μmol/L和1.0μmol/L）培养72小时。采用实时定量PCR检测Cosmc基因的表达水平。
IgAN患者中Cosmc基因的mRNA表达水平显著低于对照组（P<0.05）。RPMI1640处理后Cosmc基因表达水平显著升高（P<0.05），然而，LPS可明显逆转这一效应（P<0.05）。去甲基化修饰显著上调Cosmc基因表达（P<0.05）。
去甲基化修饰可有效逆转由外源性抑制因子导致的Comsc基因mRNA表达受抑制的情况。
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