[中国江苏省260株结核分枝杆菌分离株的间隔寡核苷酸分型（Spoligotyping）方法基因分型研究]

Liu Qiao, Xu Wei-guo, Wan Kang-lin, Wang Jian-ming, Yang Dan-dan, Lü Bing, Shao Yan, Wang Hua

Department of Epidemiology and Biostatistics, Nanjing Medical University, Nanjing, China.

Zhonghua Liu Xing Bing Xue Za Zhi. 2011 Dec;32(12):1250-4.

OBJECTIVE

To study the genotypes of Mycobacterium tuberculosis (M. tuberculosis) strains isolated from Jiangsu province and to explore the relationship between the 'Beijing family' and the drug resistance of M. tuberculosis.

METHODS

Two hundred and sixty M. tuberculosis strains were isolated from 30 drug surveillance sites in Jiangsu province. Susceptibility of the isolates to the first-line antituberculosis drugs (isoniazid, streptomycin, rifampicin and ethambutol) was tested by using the proportion method. Molecular typing of M. tuberculosis strains was determined by Spoligotyping and analyzed with BioNumerics software.

RESULTS

Based on Spoligotyping fingerprint, 260 strains showed 34 different genotypes, including 27 exclusive genotypes and 7 shared genotypes. These strains could be clustered into two groups: the Beijing family (80.4%, 209/260) and the Non-Beijing family (19.6%, 51/260). Data from logistic regression analysis revealed that infection by the Beijing family was related to an increased risk of multi-drug resistant M. tuberculosis, with the OR (95%CI) of 11.07 (1.45 - 84.50). Non-Beijing families including T1, T2, H3, H4, CAS, LAM, U and MANU2 families were also found. Among them, the CAS, LAM and MANU2 families were first reported in Jiangsu province.

CONCLUSION

It was revealed that the marked gene polymorphisms did exist in M. tuberculosis strains. The Beijing family had been the predominant strain circulating in Jiangsu province, which might be related to multi-drug resistant M. tuberculosis strains.

目的

研究从江苏省分离出的结核分枝杆菌菌株的基因型，探讨“北京家族”与结核分枝杆菌耐药性之间的关系。

方法

从江苏省30个药物监测点分离出260株结核分枝杆菌菌株。采用比例法检测这些菌株对一线抗结核药物（异烟肼、链霉素、利福平、乙胺丁醇）的敏感性。通过间隔寡核苷酸分型法对结核分枝杆菌菌株进行分子分型，并用BioNumerics软件进行分析。

结果

基于间隔寡核苷酸分型指纹图谱，260株菌株显示出34种不同的基因型，包括27种独特基因型和7种共享基因型。这些菌株可分为两组：北京家族（80.4%，209/260）和非北京家族（19.6%，51/260）。逻辑回归分析数据显示，北京家族感染与耐多药结核分枝杆菌风险增加有关，比值比（95%可信区间）为11.07（1.45 - 84.50）。还发现了包括T1、T2、H3、H4、CAS、LAM、U和MANU2家族在内的非北京家族。其中，CAS、LAM和MANU2家族在江苏省为首次报道。