McKenzie E A, Knowland J
Department of Biochemistry, Oxford, United Kingdom.
Mol Endocrinol. 1990 Jun;4(6):807-11. doi: 10.1210/mend-4-6-807.
Using DNA excess filter hybridization to pulse-labeled cellular RNA, we examined the stability of vitellogenin mRNA in Xenopus liver in relation to estrogen concentration. We showed that pharmacological concentrations of estrogen stabilize vitellogenin mRNA against degradation but that physiological concentrations do not. We concluded that there is little foundation for the common belief that estrogen stabilizes vitellogenin mRNA in normal liver cells and that such stabilization contributes to the normal expression of vitellogenin genes. We also discuss the importance of steroid concentration in other contexts, and show that the widespread tendency to use artificially high concentrations may lead to questionable conclusions.
我们使用DNA过量滤膜杂交技术对脉冲标记的细胞RNA进行检测，研究了非洲爪蟾肝脏中卵黄蛋白原mRNA的稳定性与雌激素浓度之间的关系。我们发现，药理浓度的雌激素可稳定卵黄蛋白原mRNA，使其不被降解，但生理浓度的雌激素则无此作用。我们得出结论，认为雌激素可在正常肝细胞中稳定卵黄蛋白原mRNA且这种稳定性有助于卵黄蛋白原基因正常表达的普遍观点缺乏依据。我们还讨论了类固醇浓度在其他情况下的重要性，并表明广泛使用人工高浓度类固醇可能会导致有问题的结论。
