Department of Chemistry, The Scripps Research Institute, Jupiter, Florida 33458, USA.
Org Lett. 2012 Mar 2;14(5):1302-5. doi: 10.1021/ol300187p. Epub 2012 Feb 17.
The tautomycetin (TTN) biosynthetic gene cluster has been recently cloned and sequenced from Streptomyces griseochromogenes, unveiling four genes, ttnCDFI, as candidates to encode the tailoring steps for TTN biosynthesis. It is reported that (i) TtnC plays no essential role in TTN biosynthesis, (ii) TtnI catalyzes C-5 oxidation, and (iii) combining the previous findings with TtnFD, the tailoring steps from TTN F-1 to TTN take place in the order of TtnF-catalyzed C-1"/C-2" dehydration, TtnD-catalyzed C-3" decarboxylation, and TtnI-catalyzed C-5 oxidation.
最近,从灰色链霉菌中克隆并测序了 tautomycetin(TTN)生物合成基因簇,揭示了四个基因 ttnCDFI,它们可能编码 TTN 生物合成的修饰步骤。据报道,(i)TtnC 在 TTN 生物合成中不起关键作用,(ii)TtnI 催化 C-5 氧化,(iii)结合以前的研究结果与 TtnFD,从 TTN F-1 到 TTN 的修饰步骤按 TtnF 催化的 C-1"/C-2"脱水、TtnD 催化的 C-3"脱羧和 TtnI 催化的 C-5 氧化的顺序进行。