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足月和早产临产时胎盘孕激素受体的变化。

Changes in placental progesterone receptors in term and preterm labour.

机构信息

Biosciences, Centre for Cell and Chromosome Biology, School of Health Sciences and Social Care, Brunel University, Uxbridge UB8 3PH, UK.

出版信息

Placenta. 2012 May;33(5):367-72. doi: 10.1016/j.placenta.2012.01.002. Epub 2012 Feb 16.

Abstract

Currently, preterm labour is associated with increased fetal mortality and morbidity and is often associated with elevated levels of inflammatory cytokines. However, the exact mechanisms that lead to this pathology are not fully elucidated. In the present study evidence was obtained using a specific membrane progesterone receptor (mPR) agonist, Org OD 02-0, that the progestin antagonism of apoptotic effects of a cytokine, IL-1β, on human placental BeWo cells is mediated through mPRs. Therefore the aim of this study was to determine whether the gene expression of mPRs and all other known progesterone receptors changes in human placentas at term and during labour. Quantitative PCR (qPCR) in clinical samples revealed a 2.8 fold decrease of mPRβ in labouring comparing to non-labouring tissues and 4.6 fold higher levels of mPRγ in preterm mPRγ compared to term placentas. The ratio of mPRα to PR-B was increased in term compared to preterm samples, whereas it was decreased in labour compared to non-labour placentas. There was also a high correlation between mPRα and PGRMC1 expression irrespective of pathologies. Collectively, our data indicates that changes in the ratios of progesterone receptors rather than individual fluctuations might affect progesterone signalling at the placental level.

摘要

目前,早产与胎儿死亡率和发病率增加有关,通常与炎症细胞因子水平升高有关。然而,导致这种病理的确切机制尚未完全阐明。在本研究中,使用一种特定的膜孕激素受体(mPR)激动剂 Org OD 02-0 获得了证据,孕激素对细胞因子 IL-1β 对人胎盘 BeWo 细胞凋亡作用的拮抗作用是通过 mPR 介导的。因此,本研究旨在确定 mPR 和所有其他已知孕激素受体的基因表达是否在足月和分娩时的人胎盘发生变化。临床样本的定量 PCR(qPCR)显示,与非分娩组织相比,分娩时 mPRβ 降低了 2.8 倍,与足月胎盘相比,早产 mPRγ 增加了 4.6 倍。与早产样本相比,mPRα 与 PR-B 的比值在足月时增加,而在分娩时与非分娩胎盘相比则降低。mPRα 和 PGRMC1 的表达之间也存在高度相关性,与病理无关。总之,我们的数据表明,孕激素受体比值的变化而不是单个波动可能会影响胎盘水平的孕激素信号。

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