Department of Process and Food Engineering, Faculty of Engineering, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
Bioresour Technol. 2012 Apr;110:566-71. doi: 10.1016/j.biortech.2012.01.119. Epub 2012 Feb 1.
In this paper, we report that pressed juice from oil palm frond (OPF) contained renewable sugars such as glucose, sucrose and fructose. By using a simple sugarcane press, 50% (wt/wt) of OPF juice was obtained from fresh OPF. The glucose content in the juice was 53.95±2.86g/l, which accounts for 70% of the total free sugars. We have examined the effect of various OPF juice concentrations on the production of poly(3-hydroxybutyrate), P(3HB) by Cupriavidus necator CCUG 52238(T). The cell dry mass in shake flask experiment reached 8.42g/l, with 32wt.% of P(3HB) at 30% (v/v) of OPF juice, comparable with using technical grade sugars. The biopolymer had a molecular mass, M(w) of 812kDa, with a low polydispersity index of 1.61. This result indicates that OPF juice can be used as an alternative renewable carbon source for P(3HB) production and has potential as a renewable carbon source.
本文报道了油棕棕桐（OPF）压榨汁中含有可再生物质糖，如葡萄糖、蔗糖和果糖。通过使用简单的甘蔗压榨机，从新鲜的 OPF 中可获得 50%（wt/wt）的 OPF 汁。该汁中的葡萄糖含量为 53.95±2.86g/l，占总游离糖的 70%。我们研究了不同 OPF 汁浓度对寇氏隐甲藻 CCUG 52238(T)生产聚（3-羟基丁酸酯）（P(3HB)）的影响。在摇瓶实验中，细胞干质量达到 8.42g/l，在 30%（v/v）的 OPF 汁时，P(3HB)的含量为 32wt.%，与使用工业级糖相当。该生物聚合物的分子量 M(w)为 812kDa，多分散指数为 1.61。这一结果表明，OPF 汁可用作 P(3HB)生产的替代可再生碳源，具有作为可再生碳源的潜力。
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