巴西曼氏血吸虫野鼠分离株对吡喹酮敏感性的实验研究。

Susceptibility of a Brazilian wild rodent isolate of Schistosoma mansoni to praziquantel in mice.

机构信息

Laboratory of Biology and Parasitology of Wild Mammal Reservoirs, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Av. Brasil 4365, Manguinhos, 21045-900 Rio de Janeiro, Brazil.

出版信息

Exp Parasitol. 2012 Apr;130(4):394-9. doi: 10.1016/j.exppara.2012.02.002. Epub 2012 Feb 10.

DOI:10.1016/j.exppara.2012.02.002

PMID:22343042

Abstract

The therapeutic effects of praziquantel (PZQ) against a Schistosoma mansoni isolate derived from Nectomys squamipes (isolate R) and a susceptible isolate (BH) were analyzed in Swiss mice by fecal egg counting, adult worm reduction and oogram pattern. Infected mice were orally administrated with 62.5mg/kg (group 1), 125mg/kg (group 2), 250mg/kg (group 3) and 500mg/kg (group 4), each dose divided over 3 days (49, 50 and 51 days after infection). The data were analyzed using one-way analysis of variance (ANOVA). In regard to isolate R, no fecal eggs were observed with 250 mg/Kg and 500 mg/kg (p<0.05), whereas BH excretion reached zero with all doses. Mean worm burden reduction was significantly (p<0.05) higher at the two highest concentrations, regardless of isolate. At 62.5mg/kg, the percentage of immature eggs varied from 17% (isolate R) to 38% (isolate BH). At 125 mg/kg, the percentage of immature eggs varied from 20% (isolate R) to 16% (isolate BH). At 250 mg/kg, immature eggs dropped significantly to 1% (isolate R) and 4% (isolate BH). At 500 mg/kg, no immature eggs were found in isolate R, whereas in BH was 8%. No dosage significantly (p>0.05) affected the percentage of mature eggs, regardless of isolate. There was a large increase (p<0.001) in the percentages of dead eggs in all treated groups of 62% and 64% in groups 3 and 4, respectively (isolate R). The percentage of dead eggs rose from 34% (group 1) to 58% (group 3) in isolate BH. Although group 4 showed lowest increase in the percentage of dead eggs (46%), it was higher (p<0.001) compared to the 8% in the control. Our findings indicate that the wild isolate from N. squamipes is susceptible to PZQ.

摘要

用粪便虫卵计数、成虫减少和卵图模式分析吡喹酮（PZQ）对从 Nectomys squamipes 中分离出的曼氏血吸虫分离株（分离株 R）和敏感分离株（BH）的治疗效果。感染的小鼠经口给予 62.5mg/kg（第 1 组）、125mg/kg（第 2 组）、250mg/kg（第 3 组）和 500mg/kg（第 4 组），每种剂量分 3 天服用（感染后 49、50 和 51 天）。使用单向方差分析（ANOVA）对数据进行分析。对于分离株 R，250mg/kg 和 500mg/kg 时未观察到粪便虫卵（p<0.05），而 BH 则所有剂量均达到零排泄。无论分离株如何，两个最高浓度的成虫负荷减少率均显著（p<0.05）较高。在 62.5mg/kg 时，未成熟卵的百分比从 17%（分离株 R）变化到 38%（分离株 BH）。在 125mg/kg 时，未成熟卵的百分比从 20%（分离株 R）变化到 16%（分离株 BH）。在 250mg/kg 时，未成熟卵显著减少至 1%（分离株 R）和 4%（分离株 BH）。在 500mg/kg 时，在分离株 R 中未发现未成熟卵，而在 BH 中为 8%。无论分离株如何，没有剂量显著（p>0.05）影响成熟卵的百分比。所有治疗组的死亡卵百分比均大幅增加（p<0.001），分别为 62%和 64%，在第 3 组和第 4 组（分离株 R）。在分离株 BH 中，死亡卵的百分比从 34%（第 1 组）上升至 58%（第 3 组）。尽管第 4 组显示出最低的死亡卵百分比增加（46%），但与对照组的 8%相比更高（p<0.001）。我们的研究结果表明，来自 Nectomys squamipes 的野生分离株对 PZQ 敏感。