Department of Orthodontics and Dentofacial Orthopedics, Dental School, University of Heidelberg, Im Neuenheimer Feld 400, 69120 Heidelberg, Germany.
Eur J Cell Biol. 2012 May;91(5):402-12. doi: 10.1016/j.ejcb.2011.12.005. Epub 2012 Feb 19.
Cementoblasts are cells that produce, secrete and direct the production of cementum. Resorption lacunae occur in over 90% of teeth at the compression side of the periodontal ligament and might result in an irreversible loss of the original root length. We isolated and cultivated human primary cementoblasts and investigated their expression pattern concerning markers of the osteoblastogenic differentiation (RUNX2, OCN, ALP, and BSP) and CEMP-1. Compared to osteoblasts, cementoblasts displayed an expression pattern comparable to osteoblasts in an early stage of osteoblastogenic differentiation. Next, the human primary cementoblasts were stimulated with IL-1β (1 and 10ng/ml) for 24 and 96h and subsequently subjected to compressive forces (30.3g/cm(2)) for 1 and 6h. Our in vitro data demonstrated that BSP and CEMP-1 expression significantly decreased when stimulation was accompanied by compression, while compression or stimulation alone led to increased levels of BSP and decreased levels of CEMP-1. We concluded that human primary cementoblasts subjected to compression and IL-1β stimulation impeded BSP and CEMP-1 expression, proteins that are associated with cementogenesis.
成牙骨质细胞是产生、分泌和指导牙骨质形成的细胞。在牙周韧带的受压侧，超过 90%的牙齿会出现吸收腔，这可能导致牙根长度的不可逆丧失。我们分离并培养了人原代成牙骨质细胞，并研究了它们在成骨细胞分化标志物（RUNX2、OCN、ALP 和 BSP）和 CEMP-1 方面的表达模式。与成骨细胞相比，成牙骨质细胞的表达模式与成骨细胞在成骨细胞分化的早期阶段相似。接下来，用 IL-1β（1 和 10ng/ml）刺激人原代成牙骨质细胞 24 和 96 小时，然后在 1 和 6 小时进行压缩力（30.3g/cm(2))刺激。我们的体外数据表明，当刺激伴随着压缩时，BSP 和 CEMP-1 的表达显著降低，而单独的压缩或刺激会导致 BSP 水平升高和 CEMP-1 水平降低。我们得出结论，受到压缩和 IL-1β 刺激的人原代成牙骨质细胞抑制了与牙骨质形成相关的 BSP 和 CEMP-1 的表达。
