Zeplin Philip H
Department of Trauma, Hand, Plastic and Reconstructive Surgery, Würzburg University Hospital, Oberdu¨rrbacher Str. 6, Würzburg, Germany.
Ann Plast Surg. 2012 Mar;68(3):271-5. doi: 10.1097/SAP.0b013e31824b3e06.
Burn scar formations can cause disfiguration and loss of dermal function. The purpose of this study was to examine whether application of modified silicone gel sheets with an antifibrotic drug halofuginone-eluting hybrid surface produce an effect on scar development. There were a total of 2 animal groups. The athymic nude mice (nu/nu) of both groups underwent transplantation of full-thickness human skin grafts onto their backs and setting of partial thickness burn injury. The status of local scar development was observed over a period of 3 months after the application of silicone gel sheets and also after application of surface-modified halofuginone-eluting silicone gel sheets. Subsequently, via real-time polymerase chain reaction, the cDNA levels from key mediators of scar formation (transforming growth factor beta, COL1A1, connective tissue growth factor, fibroblast growth factor 2, matrix metalloproteinase 2, matrix metalloproteinase 9) were established and statistically evaluated. In comparison with uncoated silicone gel sheets, the application of halofuginone-eluting silicone gel sheets lead to a significant difference in gene expression activity in scar tissue. Halofuginone-eluting hybrid surface silicone gel sheets significantly increase the antiscarring effect of adhesive silicone gel sheets by deceleration and downregulation of scar development by normalization of the expression activity.
烧伤瘢痕形成可导致毁容和皮肤功能丧失。本研究的目的是检验应用具有抗纤维化药物卤夫酮洗脱混合表面的改良硅胶片是否对瘢痕发展产生影响。总共分为2个动物组。两组的无胸腺裸鼠（nu/nu）均在其背部进行全层人皮肤移植并设置部分厚度烧伤损伤。在应用硅胶片后以及应用表面改性卤夫酮洗脱硅胶片后，观察3个月期间局部瘢痕发展情况。随后，通过实时聚合酶链反应，确定瘢痕形成关键介质（转化生长因子β、COL1A1、结缔组织生长因子、成纤维细胞生长因子2、基质金属蛋白酶2、基质金属蛋白酶9）的cDNA水平并进行统计学评估。与未涂层的硅胶片相比，应用卤夫酮洗脱硅胶片导致瘢痕组织中的基因表达活性存在显著差异。卤夫酮洗脱混合表面硅胶片通过使表达活性正常化来减缓并下调瘢痕发展，从而显著增强粘性硅胶片的抗瘢痕效果。
