Chen Guang-Fu, Zhang Yun-Fang, Long Qi, Wen Wen, Xu Mei-Quan, Yang Ya-Ling
Department of Pediatrics, Second People's Hospital of Shenzhen, Shenzhen, Guangdong, China.
Zhongguo Dang Dai Er Ke Za Zhi. 2012 Feb;14(2):139-43.
To study the effects of environmental enrichment on neuron proliferation, learning and memory ability and motor ability in neonatal rats with hypoxic-ischemic brain damage (HIBD).
One hundred and eight 7-day-old Sprague-Dawley rats were randomly divided into three groups: sham operation (CON group), HIBD and intervention group. HIBD model was prepared according to the classic Rice-Vannucci method. Environmental enrichment was administered for the rats in the intervention group after HIBD inducement. Behavioral tests (Water maze test, Suspension test and Slope test) were performed and the number of neural cells in the left hippocampus was examined 7, 14 and 28 days after intervention.
The pyramid cells in the hippocampus CA1 area in the HIBD group were significantly less than in the CON group at 7, 14 and 28 days (P<0.05). The number of pyramid cells in the hippocampus CA1 area in the intervention group was significantly higher than in the HIBD group (P<0.01) at 7, 14 and 28 days. The hidden platform escape latency period (EL) in the Water maze test was significantly more prolonged and the cross-platform number within 2 minutes was significantly less in the HIBD and the intervention groups than in the CON group at all observed time points (P<0.01). The EL was significantly shorter and the cross-platform number within 2 minutes was significantly higher in the intervention group than in the HIBD group at all observed time points (P<0.01). The maintain time and score in the Suspension test were significantly lower and the time in the Slope test was significantly more prolonged in the HIBD and intervention groups than in the CON group at 7, 14 and 28 days (P<0.01). An increased maintain time and score and a decreased time in the Slope test were found in the intervention group compared with the HIBD group at 14 and 28 days (P<0.01).
Environmental enrichment can improve motor function, learning and memory ability, and promote the repair and proliferation of neurons in neonatal rats with HIBD.
研究环境丰富化对缺氧缺血性脑损伤（HIBD）新生大鼠神经元增殖、学习记忆能力及运动能力的影响。
将108只7日龄Sprague-Dawley大鼠随机分为三组：假手术组（CON组）、HIBD组和干预组。按照经典的Rice-Vannucci法制备HIBD模型。对干预组大鼠在诱导HIBD后进行环境丰富化处理。在干预后7、14和28天进行行为学测试（水迷宫试验、悬挂试验和斜坡试验），并检测左侧海马神经细胞数量。
HIBD组在7、14和28天时海马CA1区锥体细胞数量显著少于CON组（P<0.05）。干预组在7、14和28天时海马CA1区锥体细胞数量显著高于HIBD组（P<0.01）。在所有观察时间点，HIBD组和干预组水迷宫试验中隐藏平台逃避潜伏期（EL）显著延长，2分钟内穿越平台次数显著少于CON组（P<0.01）。在所有观察时间点，干预组EL显著短于HIBD组，2分钟内穿越平台次数显著多于HIBD组（P<0.01）。在7、14和28天时，HIBD组和干预组悬挂试验中的维持时间和得分显著降低，斜坡试验中的时间显著延长（P<0.01）。在14和2...
