Department of Biotechnology, Yonsei University, Seoul, 120-749, Korea.
Mol Cells. 2012 Feb;33(2):203-10. doi: 10.1007/s10059-012-2271-8. Epub 2012 Jan 26.
1,2-Dibromoethane and glycidol are well known genotoxic carcinogens, which have been widely used in industry. To identify a specific biomarker for these carcinogens in cells, the cellular proteome of L5178Y mouse lymphoma cells treated with these compounds was analyzed by 2-dimensional gel electrophoresis (2-DE) and MALDI-TOF mass spectrometry (MS). Of 50 protein spots showing a greater than 1.5-fold increase or decrease in intensity compared to control cells on a 2-D gel, we focused on the candidate biomarker moesin. Western analysis using monoclonal rabbit anti-moesin confirmed the identity of the protein and its increased level of expression upon exposure to the carcinogenic compounds. Moesin expression also increased in cells treated with six additional genotoxic carcinogens, verifying that moesin could serve as a biomarker to monitor phenotypic change upon exposure to genotoxic carcinogens in L5178Y mouse lymphoma cells.
1,2-二溴乙烷和缩水甘油是众所周知的遗传毒性致癌物质，已被广泛应用于工业领域。为了鉴定这些致癌物质在细胞中的特异性生物标志物，我们通过二维凝胶电泳（2-DE）和 MALDI-TOF 质谱（MS）分析了用这些化合物处理的 L5178Y 小鼠淋巴瘤细胞的细胞蛋白质组。与对照细胞相比，在 2-DE 凝胶上有 50 个蛋白质斑点的强度增加或减少超过 1.5 倍，我们将重点放在候选生物标志物 moesin 上。使用单克隆兔抗 moesin 的 Western 分析证实了该蛋白质的身份及其在暴露于致癌化合物时表达水平的增加。在用另外六种遗传毒性致癌剂处理的细胞中，moesin 的表达也增加了，这验证了 moesin 可以作为生物标志物，用于监测 L5178Y 小鼠淋巴瘤细胞暴露于遗传毒性致癌剂时表型变化。
