Institute of Applied Biochemistry, University of Tsukuba, Tennodai 1-1-1, 305, Tsukuba, Ibaraki, Japan.
Cytotechnology. 1995 Jan;18(1-2):35-50. doi: 10.1007/BF00744318.
Production of monoclonal antibody against hepatitis B surface antigen was carried out by perfusion culture coupled with a selective removal system for ammonium ion. The removal system is composed of three sub-systems namely, cell separation by cross-flow ceramic filter, dialysis by hollow fiber module and ion-exchange by zeolite A-3 packed bed column. The ammonium ion concentration in the culture broth was effectively maintained below the inhibitory level, and the viable cell density reached 2.5×10(7) cells ml(-1) which was three times that of conventional perfusion cultures. The monoclonal antibody accumulated to a concentration as high as 26.3×10(5) mIU(-1). This is already almost half of the amount producedin vivo. The numerical investigation of the ammonium ion removal system showed the possibility to improve much more the performance of this perfusion cultivation system.
通过灌注培养与铵离子选择性去除系统的联合使用，生产出了针对乙型肝炎表面抗原的单克隆抗体。该去除系统由三个子系统组成，即通过错流陶瓷过滤器进行细胞分离、通过中空纤维模块进行透析以及通过沸石 A-3 填充床柱进行离子交换。培养物中的铵离子浓度被有效地维持在抑制水平以下，活细胞密度达到了 2.5×10(7)细胞/ml(-1)，是传统灌注培养的三倍。单克隆抗体积累到了 26.3×10(5)mIU(-1)的高浓度。这已经几乎是体内产生量的一半。对铵离子去除系统的数值研究表明，有可能进一步提高这种灌注培养系统的性能。
