Ren S M, Yang G L, Liu C Z, Zhang C X, Shou Q H, Yu S F, Li W C, Su X L
Department of Pediatrics, Affiliated Hospital, Inner Mongolia Medical College, Hohhot, Inner Mongolia, China.
Genet Mol Res. 2012 Feb 3;11(1):221-8. doi: 10.4238/2012.February.3.2.
We examined a possible association between HLA-A and -B polymorphisms and susceptibility to Henoch-Schönlein purpura (HSP) in Han and Mongolian children in Inner Mongolia, through a case-control study. Two hundred and sixty-eight unrelated children were enrolled, including 56 Mongolian and 50 Han children with HSP, 66 healthy Mongolian and 96 healthy Han children as a control group. HLA-A and -B alleles were indentified by PCR-sequence-specific oligonucleotide analysis and were further analyzed by PCR-sequencing-based typing (SBT). Frequencies of HLA-A11, HLA-B15 in Mongolian patients and HLA-A26, HLA-B35, HLA-B52 in Han patients were higher than those in the corresponding control group (P < 0.05), while frequencies of HLA-B07 and -B40 in Mongolian HSP patients were lower than those in the control group (P < 0.05). Further analysis using PCR-SBT showed that all HLA-A11 were HLA-A1101, and most HLA-B15 were HLA-B1501 in Mongolian HSP patients. All HLA-A26 were HLA-A2601 and HLA-B35 were mostly HLA-B3503 in Han patients. There were more Han patients with severe manifestations than Mongolian patients (P < 0.05). Frequencies of HLA-A26, HLA-B35 and HLA-B52 in Han patients were higher than in Mongolian patients (P < 0.05). We conclude that HLA-A11(1101) and -B15(1501) are associated with susceptibility to HSP in Mongolian children and HLA-A26(2601), HLA-B35(3503) and HLA-B52 are associated with susceptibility to HSP in Han children. HLA-B07 and -B*40 may be protective genes in Mongolian children. The different frequencies of HLA-A and -B in Mongolian and Han children may be responsible for the different manifestations in these two ethnic groups.
我们通过一项病例对照研究，调查了内蒙古汉族和蒙古族儿童中HLA - A和 - B基因多态性与过敏性紫癜（HSP）易感性之间的可能关联。共纳入268名无亲缘关系的儿童，其中包括56名蒙古族和50名汉族HSP患儿，66名健康蒙古族儿童和96名健康汉族儿童作为对照组。通过PCR - 序列特异性寡核苷酸分析鉴定HLA - A和 - B等位基因，并通过基于PCR测序的分型（SBT）进一步分析。蒙古族患者中HLA - A11、HLA - B15以及汉族患者中HLA - A26、HLA - B35、HLA - B52的频率高于相应对照组（P < 0.05），而蒙古族HSP患者中HLA - B07和 - B40的频率低于对照组（P < 0.05）。使用PCR - SBT的进一步分析表明，蒙古族HSP患者中所有的HLA - A11均为HLA - A1101，大多数HLA - B15为HLA - B1501。汉族患者中所有的HLA - A26均为HLA - A2601，大多数HLA - B35为HLA - B3503。汉族中具有严重临床表现的患者比蒙古族患者更多（P < 0.05）。汉族患者中HLA - A26、HLA - B35和HLA - B52的频率高于蒙古族患者（P < 0.05）。我们得出结论，HLA - A11（1101）和 - B15（1501）与蒙古族儿童HSP易感性相关，HLA - A26（2601）、HLA - B35（3503）和HLA - B52与汉族儿童HSP易感性相关。HLA - B07和 - B*40可能是蒙古族儿童的保护基因。蒙古族和汉族儿童中HLA - A和 - B的不同频率可能是这两个民族出现不同临床表现的原因。
