Gouget B, Rakotoambinina B, Gourmelin Y, Truchaud A
Laboratoire de biochimie, Meaux, Paris.
Rev Prat. 1990 Oct 1;40(22):2022-8.
The maintenance of a defined free H+ concentration within narrow limits is a prerequisite and feature of living organisms. In recent years the different disciplines of biological science have made considerable progress in the elucidation of the mechanisms involved in pH homeostasis. Recent advances have occurred also in the field of pH measurement. This review focuses mainly on the modern instruments for pH and blood gas analysis. The techniques of intracellular pH currently in general use are described together with some of the techniques for determination of pH in subcellular compartments.
在狭窄范围内维持特定的游离H⁺浓度是生物有机体的一个先决条件和特征。近年来,生物科学的不同学科在阐明pH稳态所涉及的机制方面取得了相当大的进展。pH测量领域也有了新的进展。本综述主要关注用于pH和血气分析的现代仪器。介绍了目前普遍使用的细胞内pH技术以及一些用于测定亚细胞区室pH的技术。