Buttle D J, Burnett D, Abrahamson M
Department of Biochemistry, Strangeways Research Laboratory, Cambridge, UK.
Scand J Clin Lab Invest. 1990 Sep;50(5):509-16. doi: 10.1080/00365519009089165.
Sputum samples from 25 patients with bronchiectasis were assayed enzymatically for myeloperoxidase, neutrophil elastase and cathepsin B, and immunologically for cystatin A, cystatin B, cystatin C, cystatin S and kininogen. High myeloperoxidase and neutrophil elastase levels were found in those sputum samples that were assessed visually to be purulent. These samples were also found to contain high levels of cathepsin B activity and cystatin A, but low levels of cystatin S and of the most effective cathepsin B inhibitor, cystatin C. In contrast, sputum samples that were low in myeloperoxidase and neutrophil elastase activities had low levels of cathepsin B and cystatin A, but high cystatin C and S levels. It is concluded that cathepsin B activity in sputum is positively correlated with the degree of inflammation and neutrophil recruitment. Although this may be due in part to reduced amounts of cathepsin B inhibitors, particularly cystatin C, theoretical considerations suggest that factors other than the gross level of inhibitors must be involved in the control of cathepsin B activity.
对25例支气管扩张症患者的痰液样本进行了髓过氧化物酶、中性粒细胞弹性蛋白酶和组织蛋白酶B的酶活性检测，以及胱抑素A、胱抑素B、胱抑素C、胱抑素S和激肽原的免疫检测。在肉眼评估为脓性的痰液样本中发现了高水平的髓过氧化物酶和中性粒细胞弹性蛋白酶。这些样本还被发现含有高水平的组织蛋白酶B活性和胱抑素A，但胱抑素S以及最有效的组织蛋白酶B抑制剂胱抑素C的水平较低。相比之下，髓过氧化物酶和中性粒细胞弹性蛋白酶活性较低的痰液样本中，组织蛋白酶B和胱抑素A的水平较低，但胱抑素C和S的水平较高。得出的结论是，痰液中的组织蛋白酶B活性与炎症程度和中性粒细胞募集呈正相关。虽然这可能部分归因于组织蛋白酶B抑制剂，尤其是胱抑素C的量减少，但理论上的考虑表明，除了抑制剂的总体水平外，其他因素也必须参与组织蛋白酶B活性的控制。
