从尼龙滤器上分离的干燥埃及血吸虫卵中提取 DNA。
DNA extraction from dried Schistosoma haematobium eggs isolated on nylon filters.
机构信息
DBL-Centre for Health Research and Development, Department of Veterinary Disease Biology, Faculty of Life Sciences, University of Copenhagen, Frederiksberg C, Denmark.
出版信息
Trans R Soc Trop Med Hyg. 2012 Apr;106(4):270-2. doi: 10.1016/j.trstmh.2011.12.002. Epub 2012 Feb 29.
Genetic studies on Schistosoma haematobium are often carried out on DNA extracted from miracidia, cercariae or adult worms. This paper presents a method for extracting DNA from S. haematobium eggs collected from urine samples and stored on nylon filters at room temperature. DNA was extracted from dried S. haematobium eggs using the DNeasy Blood & Tissue Kit (QIAGEN Sample & Assay Technologies, Copenhagen, Denmark). Selected genes were amplified using PCR to verify that DNA extraction had been successful. DNA was extracted from 45 samples and 31 had a positive PCR reaction for either or both of the two selected genes.
对埃及血吸虫的遗传研究通常是在从尾蚴、毛蚴或成虫中提取的 DNA 上进行的。本文介绍了一种从尿液样本中收集并在室温下储存在尼龙滤器上的埃及血吸虫卵中提取 DNA 的方法。使用 DNeasy Blood & Tissue Kit（QIAGEN Sample & Assay Technologies，哥本哈根，丹麦）从干燥的埃及血吸虫卵中提取 DNA。使用 PCR 扩增选定的基因，以验证 DNA 提取是否成功。从 45 个样本中提取了 DNA，其中 31 个样本对两个选定基因中的一个或两个基因的 PCR 反应均呈阳性。
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