Department of Obstetrics, Gynecology, and Reproductive Sciences, Magee-Womens Research Institute, University of Pittsburgh, 204 Craft Avenue, Pittsburgh, PA 15213, USA.
Mol Hum Reprod. 2012 Aug;18(8):417-24. doi: 10.1093/molehr/gas013. Epub 2012 Feb 29.
The largest gene cluster of human microRNAs (miRNAs), the chromosome 19 miRNA cluster (C19MC), is exclusively expressed in the placenta and in undifferentiated cells. The precise expression pattern and function of C19MC members are unknown. We sought to profile the relative expression of C19MC miRNAs in primary human trophoblast (PHT) cells and exosomes. Using high-throughput profiling, confirmed by PCR, we found that C19MC miRNAs are among the most abundant miRNAs in term human trophoblasts. Hypoxic stress selectively reduced miR-520c-3p expression at certain time-points with no effect on other C19MC miRNAs. Similarly, differentiation in vitro had a negligible effect on C19MC miRNAs. We found that C19MC miRNAs are the predominant miRNA species expressed in exosomes released from PHT, resembling the profile of trophoblastic cellular miRNA. Predictably, we detected the similar levels of circulating C19MC miRNAs in the serum of healthy pregnant women at term and in women with pregnancies complicated by fetal growth restriction. Our data define the relative expression levels of C19MC miRNAs in trophoblasts and exosomes, and suggest that C19MC miRNAs function in placental-maternal signaling.
人类 microRNAs（miRNAs）最大的基因簇，即 19 号染色体 miRNA 簇（C19MC），仅在胎盘和未分化细胞中表达。C19MC 成员的精确表达模式和功能尚不清楚。我们试图分析 C19MC miRNAs 在原代人滋养层（PHT）细胞和外泌体中的相对表达。通过高通量分析并经 PCR 验证，我们发现 C19MC miRNAs 是足月人滋养层中最丰富的 miRNAs 之一。低氧应激选择性地降低了 miR-520c-3p 在某些时间点的表达，但对其他 C19MC miRNAs 没有影响。同样，体外分化对 C19MC miRNAs 几乎没有影响。我们发现 C19MC miRNAs 是从 PHT 释放的外泌体中表达的主要 miRNA 种类，与滋养细胞 miRNA 的特征相似。可以预见的是，我们在足月健康孕妇和胎儿生长受限孕妇的血清中检测到了相似水平的循环 C19MC miRNAs。我们的数据定义了 C19MC miRNAs 在滋养层和外泌体中的相对表达水平，并表明 C19MC miRNAs 在胎盘-母体信号转导中发挥作用。
