Institute of Food Quality and Food Safety, University of Veterinary Medicine, Hannover, Hannover, Germany.
Meat Sci. 2012 Jul;91(3):272-6. doi: 10.1016/j.meatsci.2012.02.001. Epub 2012 Feb 16.
For specific production lines, European retail companies demand exclusively female pork meat. To control the quality of their suppliers the identification and a quantitative detection of the animal sex origin of the meat is therefore of importance for meat processors. To enable a fast and reliable detection of male pig meat, a real time-PCR-system was designed in the present study. This was based on the genes AMEL-X and AMEL-Y. The real time-PCR assay allowed the detection of male pig meat at a concentration of 1% yielding a detection probability of 100% while the detection probability investigating meat samples containing 0.1% male pig meat was 44.4%. The analytic sensitivity of this system was assessed to be <5 pg DNA per PCR reaction. The assessment of the accuracy of the real time-PCR assay to correctly identify sex individuals was investigated with 62 pigs including males (n=29) and females (n=33) belonging to different breeds/lines. With the newly designed test all analysed animals were correctly sexed. No amplification was obtained with cow, goat, sheep, turkey and chicken genomic DNA. The presented assay can be used for sex diagnosis, for the detection of male pig meat and for meat quality control.
对于特定的生产线，欧洲零售公司只要求女性猪肉。为了控制供应商的质量，对肉品动物性别来源的鉴定和定量检测对肉类加工商来说非常重要。为了能够快速可靠地检测公猪肉，本研究设计了一种实时 PCR 系统。该系统基于 AMEL-X 和 AMEL-Y 基因。实时 PCR 检测方法可在 1%的浓度下检测到公猪肉，检测概率为 100%，而检测含 0.1%公猪肉的肉样的检测概率为 44.4%。该系统的分析灵敏度评估为每个 PCR 反应 <5 pg DNA。使用包括 29 只雄性和 33 只雌性在内的 62 头来自不同品种/系的猪评估了实时 PCR 检测方法正确识别性别的准确性。用新设计的试验方法对所有分析动物进行了正确的性别鉴定。牛、山羊、绵羊、火鸡和鸡的基因组 DNA 均未扩增。该方法可用于性别诊断、公猪肉检测和肉品质量控制。
