CD133+CD34+和CD133+CD38+血液祖细胞作为自体外周血干细胞移植患者血小板植入的预测指标。
CD133+CD34+ and CD133+CD38+ blood progenitor cells as predictors of platelet engraftment in patients undergoing autologous peripheral blood stem cell transplantation.
作者信息
Camacho Villa Alma Y, Reyes Maldonado Elba, Montiel Cervantes Laura A, Vela Ojeda Jorge
机构信息
Departamento de Morfología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, México, DF, Mexico.
出版信息
Transfus Apher Sci. 2012 Jun;46(3):239-44. doi: 10.1016/j.transci.2012.02.002. Epub 2012 Mar 3.
BACKGROUND
Hematopoietic stem cells (HSC) have been characterized by CD34+ expression and an adequate dose of CD34+ cells is associated with a complete engraftment. CD133 is a more specific marker of HSC.
MATERIALS AND METHODS
We studied the relationship between graft content of CD34+, CD133+, and CD38+ cells and trilineage engraftment after autologous stem cell transplantation in patients with different hematological disorders. Blood samples were obtained before and after mobilization with recombinant granulocyte-colony stimulating factor (G-CSF, 16 μg/kg), from apheresis collections, and after transplantation.
RESULTS
Cell subsets were quantified by flow cytometry, and the dose of each population infused was correlated with success of engraftment. G-CSF induced mobilization of CD133+CD38+ cells (12.6-fold) and CD133+CD34+ cells (14.7-fold). A correlation was observed between the infused dose of CD133+CD34+ and CD133+CD38+ cells and platelet engraftment.
CONCLUSION
CD133+CD34+ and CD133+CD38+ cells were mobilized with G-CSF and these cell subsets were correlated with platelet engraftment.
背景
造血干细胞（HSC）已通过CD34+表达得以表征，且足够剂量的CD34+细胞与完全植入相关。CD133是HSC更具特异性的标志物。
材料与方法
我们研究了不同血液系统疾病患者自体干细胞移植后，CD34+、CD133+和CD38+细胞的移植物含量与三系植入之间的关系。在使用重组粒细胞集落刺激因子（G-CSF，16μg/kg）动员前后、单采采集时以及移植后采集血样。
结果
通过流式细胞术对细胞亚群进行定量，每个输注群体的剂量与植入成功率相关。G-CSF诱导CD133+CD38+细胞（12.6倍）和CD133+CD34+细胞（14.7倍）动员。观察到CD133+CD34+和CD133+CD38+细胞的输注剂量与血小板植入之间存在相关性。
结论
G-CSF可动员CD133+CD34+和CD133+CD38+细胞，且这些细胞亚群与血小板植入相关。
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