一种新型昆虫细胞系的特性，该细胞系源自柑橘凤蝶（鳞翅目：凤蝶科）的幼虫，并对 AcNPV 敏感。
Characterization of a new insect cell line that is derived from the neonate larvae of Papilio xuthus (Lepidoptera: Papilionidae) and its susceptibility to AcNPV.
机构信息
Research Institute of Resource Insects, Chinese Academy of Forestry, The Key Laboratory of Cultivating and Utilization of Resource Insects of State Forestry Administration, Kunming 650224, Yunnan, China.
出版信息
Tissue Cell. 2012 Jun;44(3):137-42. doi: 10.1016/j.tice.2011.11.007. Epub 2012 Mar 3.
The cell line RIRI-PX1 was established from neonate larval tissues of Papilio xuthus by performing primary cultures in the modified Grace medium that was supplemented with 20% fetal bovine serum (FBS). The cell line primarily consisted of spindle-shaped and spherical cells which attached themselves to the flask. The population-doubling times (PDTs) at the 50th and 60th passage were 42.5 h and 42.1 h respectively. The average chromosome numbers of RIRI-PX1 cell line from passage 5 to passage 50 ranged from 103 to 199. It was confirmed that RIRI-PX1 cell line was derived from P. xuthus by comparing the mitochondrial cytochrome c oxidase subunit I gene (COI) of RIRI-PX1 cells and P. xuthus eggs. This cell line was susceptible to the Autographa californica nucleopolyhedrovirus (AcNPV) and produced high yield of polyhedral occlusion bodies (43.9OBs/cell) after 10 days of infection by AcNPV. The virus titer of AcNPV infected RIRI-PX1 cells was 3.25×10⁷ TCID₅₀/ml. We concluded that the RIRI-PX1 cell line is established from the neonate larvae tissues successfully and the cells of the cell line are sensitive to AcNPV.
细胞系 RIRI-PX1 是通过在改良的 Grace 培养基中进行原代培养，并用 20%胎牛血清（FBS）进行补充，从 Papilio xuthus 的新生儿幼虫组织中建立的。该细胞系主要由附着在瓶壁上的纺锤形和球形细胞组成。第 50 代和第 60 代的倍增时间（PDT）分别为 42.5 小时和 42.1 小时。RIRI-PX1 细胞系从第 5 代到第 50 代的平均染色体数从 103 到 199 不等。通过比较 RIRI-PX1 细胞和 P. xuthus 卵的线粒体细胞色素 c 氧化酶亚基 I 基因（COI），证实 RIRI-PX1 细胞系来源于 P. xuthus。该细胞系对美洲棉铃虫核多角体病毒（AcNPV）敏感，在感染 AcNPV 10 天后产生 43.9OBs/细胞的高产量多面体封闭体。感染 AcNPV 的 RIRI-PX1 细胞的病毒滴度为 3.25×10⁷ TCID₅₀/ml。我们得出结论，RIRI-PX1 细胞系是从新生儿幼虫组织中成功建立的，并且该细胞系的细胞对 AcNPV 敏感。
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