利用亚临界水预处理从解脂耶氏酵母 Po1g 生物质中最大化生产生物柴油。
Maximizing biodiesel production from Yarrowia lipolytica Po1g biomass using subcritical water pretreatment.
机构信息
Department of Chemical Engineering, National Taiwan University of Science and Technology, 43 Keelung Rd., Sec.4, Taipei 106-07, Taiwan.
出版信息
Bioresour Technol. 2012 May;111:201-7. doi: 10.1016/j.biortech.2012.02.052. Epub 2012 Feb 22.
The yeast Yarrowia lipolytica Po1g is one of the oleaginous microorganisms with a potential for biodiesel production. Sub-critical water (SCW) treatment has been known as an effective method for increasing the amount of extractable lipids in microorganisms. In this work, the amount of neutral lipids and fatty acid profiles in neutral lipids extracted from Y. lipolytica Po1g with and without SCW pre-treatment were investigated. The effects of temperature (125, 150 or 175°C), amount of water (20, 30 or 40 mL/g biomass) and time (10, 20 or 30 min) showed that maximum neutral lipid (42.69%, w/w) could be achieved at 175°C using 20 mL water for 20 min. The maximum neutral lipid from unpretreated samples was 23.21%. No difference in fatty acid profiles was observed, but long chain fatty acids were observed in higher amount in SCW pretreated samples. SCW pretreatment increased biodiesel yield twofold.
产油脂酵母假丝酵母 Po1g 是具有生产生物柴油潜力的微生物之一。亚临界水（SCW）处理已被证实是增加微生物中可提取脂质含量的有效方法。在这项工作中，研究了未经和经 SCW 预处理的假丝酵母 Po1g 中中性脂质的中性脂质含量和脂肪酸分布。考察了温度（125、150 或 175°C）、水量（20、30 或 40 mL/g 生物质）和时间（10、20 或 30 min）的影响，结果表明，在 175°C 下使用 20 mL 水处理 20 min 可获得最大中性脂质（42.69%，w/w）。未经预处理样品的最大中性脂质为 23.21%。脂肪酸分布没有差异，但在经 SCW 预处理的样品中观察到长链脂肪酸的含量更高。SCW 预处理使生物柴油产率提高了一倍。
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