KTH Royal Institute of Technology, School of Chemical Science and Engineering, Department of Chemistry, Analytical Chemistry, Stockholm, Sweden.
Anal Biochem. 2012 Jun 1;425(1):18-20. doi: 10.1016/j.ab.2012.02.039. Epub 2012 Mar 6.
Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is widely used for analysis of macromolecules like peptides and proteins. The analysis procedure is generally simple but must be adapted to the characteristics of the analytes. Therefore, specific matrices suitable for, e.g., hydrophobic proteins and peptides that are difficult to analyze would be preferable in order to optimize the outcome. In the present work, 2,6-dihydroxyacetophenone (DHAP) was shown to be beneficial in comparison to DHB for intact bacteriorhodopsin (BR) as well as for chemically digested BR.
基质辅助激光解吸电离质谱（MALDI-MS）广泛用于分析多肽和蛋白质等大分子。分析过程通常很简单，但必须适应分析物的特性。因此，例如对于疏水性蛋白质和肽等难以分析的物质，使用特定的基质会更有利，以便优化结果。在本工作中，与 DHB 相比，2,6-二羟基苯乙酮（DHAP）对于完整的菌紫质（BR）以及化学消化的 BR 都更有益。
