Zhang Ying, Li Bao-Zhen, Yang Jin-Shui, Wang Shuang-Qing, Yuan Hong-Li
College of Biological Sciences, China Agricultural University, Beijing 100193, China.
Huan Jing Ke Xue. 2012 Jan;33(1):299-304.
Butane-oxidizing bacteria in soil sample sites from Puguang gas field in Sichuan province and Jianghan oil field in Hubei province were isolated and 16S rRNA gene sequence and phylogenetic analysis were applied. The differences of number and phylogenetic position and population diversity of hydrocarbon-oxidizing bacteria in different environment were investigated. The results show that 25 strains of butane-oxidizing bacteria were isolated. Based on sequencing of 16S rRNA gene, the species of bacteria in two samples are classified into 3 phyla including Firmicutes, Actinobacteria and Proteobacteria. The community structure of butane-oxidizing bacteria isolated from two oil samples is simple, both of them contain 4 genus including Bacillus, Pseudomonas, Rhodococcus and Arthrobacter. Strains in the genus of Ochrobactrum and Mycobacterium were only isolated from Puguang gas field. The number and population diversity of butane-oxidizing bacteria in Puguang gas field was more than those in Jianghan oil field.
从四川省普光气田和湖北省江汉油田的土壤采样点分离出丁烷氧化细菌，并对其进行16S rRNA基因序列测定和系统发育分析。研究了不同环境中烃氧化细菌数量、系统发育位置及种群多样性的差异。结果表明，共分离出25株丁烷氧化细菌。基于16S rRNA基因测序，两个样品中的细菌种类被分为厚壁菌门、放线菌门和变形菌门3个门。从两个油样中分离出的丁烷氧化细菌群落结构简单，均含有芽孢杆菌属、假单胞菌属、红球菌属和节杆菌属4个属。慢生根瘤菌属和分枝杆菌属的菌株仅从普光气田分离得到。普光气田丁烷氧化细菌的数量和种群多样性均高于江汉油田。
