Bagramian K A, Martirosov S M
Biofizika. 1990 Jul-Aug;35(4):624-7.
The importance of delta mu H+ for transport of K+ via K(+)-ionophore and H(+)-K(+)-pump was studied. It was shown that the operation of the pump was decelerated by oxidant ferrycyanide, whereas sulfhydryl reagent dithiothreitol (DTT) drastically accelerated ATP driven ion exchange. Introduction of protonophore CCCP into the medium completely blocked the pump operation. However, the addition of DTT after CCCP restored the high level activity of the pump. At the same time DTT was unable to restore K+ accumulation after CCCP in aerobically grown bacteria for which the K+ uptake was performed across the electrical field gradient. Thus it was established that delta mu H+ was necessary for ATP driven ionic systems as a regulator of the membrane redox state.
研究了△μH⁺对钾离子通过钾离子载体和氢钾泵转运的重要性。结果表明，氧化剂铁氰化物会使泵的运转减速，而巯基试剂二硫苏糖醇（DTT）则会显著加速ATP驱动的离子交换。向培养基中加入质子载体CCCP会完全阻断泵的运转。然而，在加入CCCP后再添加DTT可恢复泵的高水平活性。同时，对于需氧生长的细菌，在加入CCCP后DTT无法恢复钾离子的积累，这些细菌的钾离子摄取是通过电场梯度进行的。因此确定，△μH⁺作为膜氧化还原状态的调节剂，对于ATP驱动的离子系统是必需的。
