Luo Xiao-Fei, Yang Yuan, Sun Cheng-Jun
Department of Sanitary Technology, West China School of Public Health, Sichuan University, Chengdu 610041, China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2012 Jan;43(1):108-12.
To develop a method for the simultaneous determination of 44 organophosphorus pesticides in food by SPE disk extraction-capillary gas chromatography with pulsed flame photometric detection.
Organophosphorus pesticides in food were extracted ultrasonically with water. Then the extract was cleaned-up with SPE disk and eluted with ethyl acetate. Finally the eluent was condensed to 1mL under N2 at 55 degrees C. Gas chromatography was applied for quantitative detection of the organophosphorus pesticides in the sample.
The linear range of the method for all the pesticides were in the range of 0.01-0.5 mg/kg with correlation coefficients of 0.992-1.000. The detection limits of the method were in the range of 0.0005-0.01 mg/kg. The recoveries for most pesticides were 60%-120% with relative standard deviations of less than 15%.
The method is simple, sensitive, environmentally friendly and suitable for the determination of organophosphorous pesticides in food.
建立一种采用固相萃取圆盘萃取-毛细管气相色谱-脉冲火焰光度检测法同时测定食品中44种有机磷农药的方法。
食品中的有机磷农药用水超声提取。然后提取物用固相萃取圆盘净化，并用乙酸乙酯洗脱。最后，洗脱液在55℃下用氮气浓缩至1mL。采用气相色谱法定量检测样品中的有机磷农药。
该方法对所有农药的线性范围为0.01 - 0.5mg/kg，相关系数为0.992 - 1.000。该方法的检测限为0.0005 - 0.01mg/kg。大多数农药的回收率为60% - 120%，相对标准偏差小于15%。
该方法简便、灵敏、环保，适用于食品中有机磷农药的测定。
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