de Mello Ramon Andrade, Ferreira Mónica, Costa Sandra, Costa Bruno Marques, Pires Filipa Soares, Neves Inês, Almeida Maria Inês, Cunha João, Oliveira Pedro, Hespanhol Venceslau, Reis Rui Manuel
Department of Medicine, São João Hospital, Faculty of Medicine, University of Porto, Alameda Prof. Hernani Monteiro, 4200-319, Porto, Portugal.
Tumour Biol. 2012 Oct;33(5):1341-8. doi: 10.1007/s13277-012-0382-7. Epub 2012 Mar 29.
Epidermal growth factor (EGF) and its receptor play critical roles in non-small cell lung cancer (NSCLC) carcinogenesis. A functional polymorphism in the EGF gene has been linked to increased cancer susceptibility. This study aimed to evaluate the role of the EGF +61A/G polymorphism as risk factors in NSCLC patients. For the present case-control study, we analyzed 112 NSCLC and 126 cancer-free controls from Portugal. Following DNA isolation from peripheral blood, EGF +61A/G polymorphism was assessed by polymerase chain reaction-restriction fragment length polymorphism. Univariate and multivariate logistic regression analyses were used to calculate odds ratio (OR) and 95 % confidence intervals (95 % CI). False-positive report probability was also assessed. The EGF +61 genotypes frequencies in NSCLC were AA (23.2 %), AG (51.8 %), and GG (25 %) and in controls, AA (40.5 %), AG (41.3 %), and GG (18.3 %). When compared to the reference genotype (EGF +61A/A), we found a statistically significant association between EGF +61 A/G (OR = 2.142, 95 % CI 1.170-3.924) and EGF +61G/G (OR = 2.398, 95 % CI 1.157-4.968) genotypes and susceptibility to development of NSCLC. Furthermore, stratification by sex revealed a trend to increased risk of males carrying +61A/G genotype for developing NSCLC (OR = 2.044, 95 % CI 0.998-4.188) when compared to A/A genotype. Our data suggest an increased risk to develop NSCLC in Portuguese population carrying the EGF +61A/G and +61G/G genotypes.
表皮生长因子（EGF）及其受体在非小细胞肺癌（NSCLC）的致癌过程中发挥着关键作用。EGF基因的一种功能性多态性与癌症易感性增加有关。本研究旨在评估EGF +61A/G多态性作为NSCLC患者风险因素的作用。在本病例对照研究中，我们分析了来自葡萄牙的112例NSCLC患者和126例无癌对照。从外周血中分离DNA后，通过聚合酶链反应-限制性片段长度多态性评估EGF +61A/G多态性。使用单因素和多因素逻辑回归分析来计算比值比（OR）和95%置信区间（95%CI）。还评估了假阳性报告概率。NSCLC患者中EGF +61基因型频率为AA（23.2%）、AG（51.8%）和GG（25%），对照中为AA（40.5%）、AG（41.3%）和GG（18.3%）。与参考基因型（EGF +61A/A）相比，我们发现EGF +61 A/G（OR = 2.142，95%CI 1.170 - 3.924）和EGF +61G/G（OR = 2.398，95%CI 1.157 - 4.968）基因型与NSCLC发生易感性之间存在统计学显著关联。此外，按性别分层显示，与A/A基因型相比，携带+61A/G基因型的男性患NSCLC的风险有增加趋势（OR = 2.044，95%CI 0.998 - 4.188）。我们的数据表明，携带EGF +61A/G和+61G/G基因型的葡萄牙人群患NSCLC的风险增加。
