Attia Mai Shafik, Shoreibah Eatemad A, Ibrahim Samir A, Nassar Hamdy A
Department of Oral Medicine, Periodontology, Oral Diagnosis and Radiology, Faculty of Dental Medicine-Girls' Branch, Al Azhar University, Cairo, Egypt.
J Int Acad Periodontol. 2012 Jan;14(1):7-16.
The aim of this study was to histologically evaluate the effectiveness of different times of initiating orthodontic tooth movement on enhancement of bone formation in surgical bony defects.
In 18 male guinea pigs, 3-4 months of age, a bony defect was created in the alveolar process midway between the central incisor and mandibular 1st molar. These bony defects were implanted with bioactive glass particles and collagen membrane. According to the application of the orthodontic tooth movement, the animals were divided into three groups, each comprised of six guinea pigs. In Group I, the orthodontic tooth movement was initiated immediately after the surgical procedure. In Group II, the orthodontic tooth movement was applied 2 weeks after the surgical procedure, while in Group III no orthodontic tooth movement was applied. Section blocks for histology were made at 1, 3 and 6 weeks after the surgical procedure.
All experimental sites showed active bone formation with plump osteoblast and osteoid matrix deposition in the treated area. In Groups I and II a dense fibrous tissue formation and highly cellular coarse bone were seen at six weeks. The histomorphometric analysis showed that Group I revealed the greatest number of newly formed trabeculae: 2.4, 6.4 and 8.6 at 1, 3 and 6 weeks, respectively. In addition, Group I defects revealed a greater total surface area of newly formed bone than Groups I and III: 2.96 mm2 at the end of the study period.
The combined orthodontic/regenerative therapy seemed to enhance the process of bone formation. Bone formation was histologically observed in all test groups. Defects treated with immediate application of orthodontic tooth movement showed a statistically significant increase in trabecular count and total surface area of newly formed bone than the other experimental groups.
本研究旨在通过组织学方法评估不同时间开始正畸牙齿移动对手术造成的骨缺损处骨形成增强效果的影响。
选取18只3 - 4月龄的雄性豚鼠，在中切牙与下颌第一磨牙之间的牙槽突处制造骨缺损。这些骨缺损处植入生物活性玻璃颗粒和胶原膜。根据正畸牙齿移动的应用情况，将动物分为三组，每组6只豚鼠。第一组在手术后立即开始正畸牙齿移动。第二组在手术后2周进行正畸牙齿移动，而第三组不进行正畸牙齿移动。在手术后1、3和6周制作组织学切片。
所有实验部位在治疗区域均显示出活跃的骨形成，有成骨细胞饱满和类骨质基质沉积。在第一组和第二组中，六周时可见致密的纤维组织形成和细胞丰富的粗大骨。组织形态计量学分析显示，第一组新形成的小梁数量最多：在1、3和6周时分别为2.4、6.4和8.6。此外，第一组缺损处新形成骨的总表面积大于第二组和第三组：在研究期末为2.96平方毫米。
正畸/再生联合治疗似乎能增强骨形成过程。所有测试组在组织学上均观察到骨形成。与其他实验组相比，立即进行正畸牙齿移动治疗的缺损处小梁计数和新形成骨的总表面积在统计学上有显著增加。
