Department of Biological Science and Engineering, Institute of Mitochondrial Biology and Medicine, The Key Laboratory of Biomedical Information Engineering of Ministry of Education, Xi'an Jiaotong University School of Life Science and Technology, Xi'an 710049, China.
Phytomedicine. 2012 Jun 15;19(8-9):672-6. doi: 10.1016/j.phymed.2012.03.004. Epub 2012 Apr 4.
Paeoniae radix is a traditional Chinese medicinal herb for treating some diseases; important components are total paeony glycosides (TPGs), an approved drug by the State Food and Drug Administration (SFDA) for the therapy of rheumatoid arthritis (RA). We firstly reported myocardial benefits of TPGs previously, and the present study is to further investigate the underlying mechanisms for preventing oxidative damage in cardiomyopathy. We measured the capacity of TPGs to scavenge free radicals in vitro. Then 60 SD rats were randomly divided into five groups: (1) a normal control group, (2) an isoprenaline (ISO)-induced myocardial ischemic model group, (3) a TPG treatment group (TPGs 269.4 mg/kg delivered by intragastric administration for 3 days before ISO administration and TPGs 449 mg/kg delivered for 3 days after ISO administration), (4) a TPG therapy group (TPGs 449 mg/kg delivered for 3 days after ISO administration), and (5) a positive control group (propranolol 15 mg/kg for 3 days after ISO administration). The ISO-induced myocardial ischemic model was established by subcutaneous injection of 1mg/kg/8h ISO (2 times). The activities of myocardial enzymes, including glutamic oxaloacetic transaminase (GOT), creatine kinase (CK), lactate dehydrogenase (LDH), antioxidant enzyme superoxide dismutase (SOD) as well as the content of lipid peroxidation product malondialdehyde (MDA) were detected. We found that TPGs potently eliminated hydroxyl radicals and superoxide in vitro using ESR assays. Compared with model rats, TPG treatment, TPG therapy and the positive control treatment exhibited significantly reduced activities of GOT, LDH, and CK (p < 0.01), increased activity of SOD (p < 0.01) and lower levels of MDA (p < 0.05). More interestingly, the protective effect of TPG treatment was even better than that of propranolol. These results suggest that TPGs significantly ameliorate ISO-induced myocardial ischemia and their action might be through reducing oxidative stress in ischemic myocardium.
白芍是一种传统的中药，用于治疗某些疾病；其重要成分是总芍药苷（TPG），它是国家食品药品监督管理局（SFDA）批准用于治疗类风湿关节炎（RA）的药物。我们之前首次报道了 TPG 对心肌的益处，本研究旨在进一步研究其预防心肌病氧化损伤的潜在机制。我们测量了 TPG 清除自由基的能力。然后，将 60 只 SD 大鼠随机分为五组：（1）正常对照组；（2）异丙肾上腺素（ISO）诱导的心肌缺血模型组；（3）TPG 治疗组（ISO 给药前 3 天通过灌胃给予 TPG269.4mg/kg，ISO 给药后 3 天给予 TPG449mg/kg）；（4）TPG 治疗组（ISO 给药后 3 天给予 TPG449mg/kg）；（5）阳性对照组（ISO 给药后 3 天给予普萘洛尔 15mg/kg）。通过皮下注射 1mg/kg/8h ISO（2 次）建立 ISO 诱导的心肌缺血模型。检测心肌酶，包括谷草转氨酶（GOT）、肌酸激酶（CK）、乳酸脱氢酶（LDH）、抗氧化酶超氧化物歧化酶（SOD）的活性以及脂质过氧化产物丙二醛（MDA）的含量。我们发现 TPG 能够通过 ESR 测定在体外有效清除羟自由基和超氧自由基。与模型大鼠相比，TPG 治疗、TPG 治疗和阳性对照治疗显著降低 GOT、LDH 和 CK 的活性（p<0.01），提高 SOD 的活性（p<0.01）并降低 MDA 的水平（p<0.05）。更有趣的是，TPG 治疗的保护作用甚至优于普萘洛尔。这些结果表明 TPG 显著改善 ISO 诱导的心肌缺血，其作用可能是通过减轻缺血心肌的氧化应激。
