通过荧光共振能量转移显微镜成像蛋白质相互作用：荧光寿命成像测量
Imaging protein interactions by FRET microscopy: FLIM measurements.
作者信息
Verveer Peter J, Rocks Oliver, Harpur Ailsa G, Bastiaens Philippe I H
出版信息
CSH Protoc. 2006 Nov 1;2006(6):pdb.prot4599. doi: 10.1101/pdb.prot4599.
PMID:22485986
Abstract
This image acquisition protocol is a basic plan for taking a fluorescence lifetime imaging (FLIM) series. FLIM makes live-cell FRET measurements based only on donor fluorescence more feasible, because lifetimes are independent of probe concentration and light path length. The former is not easy to determine in cells, and the latter means that cell shape is not a factor.
摘要
此图像采集协议是获取荧光寿命成像（FLIM）系列的基本方案。由于荧光寿命与探针浓度和光程长度无关，FLIM使得仅基于供体荧光进行活细胞荧光共振能量转移（FRET）测量变得更加可行。前者在细胞中不易确定，而后者意味着细胞形状不是一个影响因素。
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