Department of Hematology, Dr. José E. González University Hospital of the School of Medicine of the Autonomous University of Nuevo Leon, Monterrey, Mexico.
Transfusion. 2012 Dec;52(12):2606-13. doi: 10.1111/j.1537-2995.2012.03638.x. Epub 2012 Apr 4.
Umbilical cord blood (UCB) represents an alternative source of stem cells for transplantation for the treatment of hematologic malignancies and genetic disorders. There is scarce information detailing cord blood bank (CBB) collection and transplantation activities from developing countries. We documented our experience at a public university hospital in northeast Mexico.
We carried out a retrospective and descriptive analysis of our CBB activity during an 8-year period from May 2002 to September 2010. Collection, processing, and cryopreservation of CB were carried out following standard operating procedures. The minimum volume and total nucleated cell (TNC) content for cryopreservation were 80 mL and 8.0 × 10(8) , respectively.
A total of 1256 UCB units were collected; 428 (34%) were banked and 828 (66%) were discarded. The main reason for exclusion was biologic: low volume and/or low number of TNC accounted for 84% of the total discarded units. Cryopreserved cord blood units (CBUs) had a median volume of 113.8 mL (range, 80-213.2 mL) and 13.0 × 10(8) (range, 8 × 10(8) -36.6 × 10(8) ) TNCs. Cell viability was 99.3% (88-100%). The median CD34+ cell content was 4.0 × 10(6) (0.46 × 10(6) -19.38 × 10(6) ). Sixteen units have been released for transplantation, leading to a utilization rate of 3.7%.
CBB demands considerable human and financial resources; it is then essential for centers at developing countries to share their experience, results, and databases to increase the probability of finding matching units for their patients. Efforts to create and maintain CBBs allow to offer this therapeutic option at an affordable cost.
脐带血（UCB）是治疗血液系统恶性肿瘤和遗传疾病的移植替代干细胞来源。关于发展中国家脐血库（CBB）采集和移植活动的详细信息很少。我们记录了我们在墨西哥东北部一家公立大学医院的经验。
我们对 2002 年 5 月至 2010 年 9 月 8 年间的 CBB 活动进行了回顾性和描述性分析。按照标准操作规程进行 CB 的采集、处理和冷冻保存。冷冻保存的最小体积和总核细胞（TNC）含量分别为 80 mL 和 8.0×10(8)。
共采集了 1256 份 UCB 单位，其中 428 份（34%）被保存，828 份（66%）被丢弃。排除的主要原因是生物学方面的：低体积和/或低 TNC 数量占总丢弃单位的 84%。冷冻保存的脐带血单位（CBU）的中位数体积为 113.8 mL（范围 80-213.2 mL）和 13.0×10(8)（范围 8×10(8) -36.6×10(8)）TNC。细胞活力为 99.3%（88-100%）。中位数 CD34+细胞含量为 4.0×10(6)（0.46×10(6) -19.38×10(6)）。已释放 16 个单位进行移植，利用率为 3.7%。
CBB 需要大量的人力和财力资源；因此，发展中国家的中心必须分享他们的经验、结果和数据库，以增加为患者找到匹配单位的可能性。努力建立和维持 CBB 可以以可承受的成本提供这种治疗选择。
